Transgenic Research

, Volume 17, Issue 5, pp 769–782

Transplastomic tobacco plants expressing a fatty acid desaturase gene exhibit altered fatty acid profiles and improved cold tolerance

  • Wendy Craig
  • Paolo Lenzi
  • Nunzia Scotti
  • Monica De Palma
  • Paola Saggese
  • Virginia Carbone
  • Noreen McGrath Curran
  • Alan M. Magee
  • Peter Medgyesy
  • Tony A. Kavanagh
  • Philip J. Dix
  • Stefania Grillo
  • Teodoro Cardi
Original Paper

DOI: 10.1007/s11248-008-9164-9

Cite this article as:
Craig, W., Lenzi, P., Scotti, N. et al. Transgenic Res (2008) 17: 769. doi:10.1007/s11248-008-9164-9

Abstract

The possibility of altering the unsaturation level of fatty acids in plant lipids by genetic transformation has implications for the stress tolerance of higher plants as well as for their nutritional value and industrial utilisation. While the integration and expression of transgenes in the plastome has several potential advantages over nuclear transformation, very few attempts have been made to manipulate fatty acid biosynthesis using plastid transformation. We produced transplastomic tobacco plants that express a Δ9 desaturase gene from either the wild potato species Solanum commersonii or the cyanobacterium Anacystis nidulans, using PEG-mediated DNA uptake by protoplasts. Incorporation of chloroplast antibiotic-insensitive point mutations in the transforming DNA was used to select transformants. The presence of the transcript and the Δ9 desaturase protein in transplastomic plants was confirmed by northern and western blot analyses. In comparison with control plants, transplastomic plants showed altered fatty acid profiles and an increase in their unsaturation level both in leaves and seeds. The two transgenes produced comparable results. The results obtained demonstrate the feasibility of using plastid transformation to engineer lipid metabolic pathways in both vegetative and reproductive tissues and suggest an increase of cold tolerance in transplastomic plants showing altered leaf fatty acid profiles. This is the first example of transplastomic plants expressing an agronomically relevant gene produced with the “binding-type” vectors, which do not contain a heterologous marker gene. In fact, the transplastomic plants expressing the S. commersonii gene contain only plant-derived sequences, a clear attraction from a public acceptability perspective.

Keywords

Plastid transformationStress toleranceMetabolic engineeringTobaccoLipid metabolism

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Wendy Craig
    • 1
  • Paolo Lenzi
    • 1
  • Nunzia Scotti
    • 1
  • Monica De Palma
    • 1
  • Paola Saggese
    • 2
  • Virginia Carbone
    • 2
  • Noreen McGrath Curran
    • 3
  • Alan M. Magee
    • 4
  • Peter Medgyesy
    • 3
  • Tony A. Kavanagh
    • 4
  • Philip J. Dix
    • 3
  • Stefania Grillo
    • 1
  • Teodoro Cardi
    • 1
  1. 1.CNR-IGV, Institute of Plant Genetics-PorticiPorticiItaly
  2. 2.CNR-ISA, Institute of Food ScienceAvellinoItaly
  3. 3.Plant Cell Biology Laboratory, Biology DepartmentNUI-MaynoothMaynoothIreland
  4. 4.Plant Molecular Biology LaboratorySmurfit Institute of Genetics, Trinity CollegeDublin 2Ireland