Transgenic Research

, Volume 17, Issue 2, pp 171–180

Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II

Authors

    • Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology
  • Sudesh Kumar Yadav
    • Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology
    • Biotechnology DivisionInstitute of Himalayan Bioresource Technology
  • Ashwani Pareek
    • Stress Physiology and Molecular Biology Laboratory, School of Life SciencesJawaharlal Nehru University
  • M. K. Reddy
    • Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology
  • S. K. Sopory
    • Plant Molecular Biology GroupInternational Centre for Genetic Engineering and Biotechnology
Original Paper

DOI: 10.1007/s11248-007-9082-2

Cite this article as:
Singla-Pareek, S.L., Yadav, S.K., Pareek, A. et al. Transgenic Res (2008) 17: 171. doi:10.1007/s11248-007-9082-2

Abstract

Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.

Keywords

Functional validationGlyoxalase II overexpressionMethylglyoxalRice transgenicsSalinity tolerance

Copyright information

© Springer Science+Business Media B.V. 2007