Original Paper

Transgenic Research

, Volume 17, Issue 2, pp 171-180

Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II

  • Sneh L. Singla-PareekAffiliated withPlant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology Email author 
  • , Sudesh Kumar YadavAffiliated withPlant Molecular Biology Group, International Centre for Genetic Engineering and BiotechnologyBiotechnology Division, Institute of Himalayan Bioresource Technology
  • , Ashwani PareekAffiliated withStress Physiology and Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University
  • , M. K. ReddyAffiliated withPlant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology
  • , S. K. SoporyAffiliated withPlant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology

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Abstract

Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.

Keywords

Functional validation Glyoxalase II overexpression Methylglyoxal Rice transgenics Salinity tolerance