Transgenic Research

, Volume 16, Issue 4, pp 515–525

Silencing of chaperonin 21, that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits

  • Uri Hanania
  • Margarita Velcheva
  • Etti Or
  • Moshe Flaishman
  • Nachman Sahar
  • Avihai Perl
Original Paper

DOI: 10.1007/s11248-006-9044-0

Cite this article as:
Hanania, U., Velcheva, M., Or, E. et al. Transgenic Res (2007) 16: 515. doi:10.1007/s11248-006-9044-0

Abstract

Vitis vinifera L. cv. ‘Thompson Seedless’ presents a type of stenospermocarpy in grape where fertilization occurs but seeds abort and fail to develop. To unravel the molecular basis for stenospermocarpy in grapes, subtractive hybridization was carried out in order to isolate differentially regulated genes that participate in the seedlessness machinery. Two ‘Thompson’ lines, a seeded and a seedless, were screened during different flower developmental stages. One of the genes, that was differentially expressed between the seeded and seedless lines, was the chloroplast chaperonin 21 (ch-Cpn21). ch-Cpn21 is a 21-kDa co-chaperonin polypeptide formed by two GroES-like domains fused together in tandem. Silencing of ch-Cpn21 in Nicotianabenthamiana plants resulted in leaf stunting, chlorosis, as well as ovary necrogenesis leading to seed abortion. Moreover, organ-specific silencing of ch-Cpn21 only in Lycopersicum esculentum fruits resulted in the development of seedless tomatoes. These results suggest that ch-Cpn21 may play a role in seed abortion in stenospermocarpic grapes.

Keywords

Vitis vinifera Tobacco rattle virus (TRV) Seed abortion Chaperonin 21 Tomato fruit agro-injection 

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Uri Hanania
    • 1
  • Margarita Velcheva
    • 1
    • 2
  • Etti Or
    • 1
  • Moshe Flaishman
    • 1
  • Nachman Sahar
    • 1
  • Avihai Perl
    • 1
  1. 1.Department of Fruit Tree SciencesInstitute of Plant Sciences, Agricultural Research OrganizationBet-DaganIsrael
  2. 2.Alberta Research CouncilVegrevilleCanada

Personalised recommendations