Transgenic Research

, Volume 15, Issue 3, pp 271–275

Transgenic RNAi: Accelerating and Expanding Reverse Genetics in Mammals

Perspective

DOI: 10.1007/s11248-006-0023-2

Cite this article as:
Xia, XG., Zhou, H. & Xu, Z. Transgenic Res (2006) 15: 271. doi:10.1007/s11248-006-0023-2

Abstract

Reverse genetics in mammals has relied on gene targeting strategies and has mostly been limited to the mouse. Gene targeting through homologous recombination in mouse ES cells has drawbacks which include time, expense and complexity. Recently, a new approach has been developed based on RNA-interference (RNAi) which is simpler, faster, less expensive, and should be applicable to mammalian species other than mouse. The advent of RNAi is poised to accelerate the pace at which reverse genetics can be applied to study gene function in mammals.

Keywords

RNAi Transgenic Gene silencing miRNA Knockdown Mouse 

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular PharmacologyUniversity of Massachusetts Medical SchoolWorcesterUSA

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