Original Paper

Transgenic Research

, Volume 15, Issue 4, pp 455-463

Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

  • Octavio Guerrero-AndradeAffiliated withDepartamento de Ingeniería Genética de Plantas, Cinvestav Campus Guanajuato
  • , Elizabeth Loza-RubioAffiliated withCentro Nacional de Investigaciones Disciplinarias en Microbiología Animal, INIFAP. Carretera Federal
  • , Teresa Olivera-FloresAffiliated withFacultad de Química, Conjunto E, Laboratorio 116, Paseo de la Investigación Científica, Universidad Nacional Autónoma de México
  • , Tamás Fehérvári-BoneAffiliated withDepartamento de Producción Animal: Aves. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México
  • , Miguel Angel Gómez-LimAffiliated withDepartamento de Ingeniería Genética de Plantas, Cinvestav Campus Guanajuato Email author 

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Abstract

Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protein in maize kernels. The presence of the transgene was verified by Southern and western blots. Feeding chickens with kernels containing the F protein induced the production of antibodies, which conferred protection against a viral challenge. This protection was comparable to that conferred by a commercial vaccine. Possible uses of this plant-based F protein as a potential mucosal vaccine are discussed.

Keywords

Transgenic plants Recombinant proteins Maize Newcastle disease virus Chickens