Transgenic Research

, Volume 14, Issue 4, pp 417–428

Increased transgene integration efficiency upon microinjection of DNA into both pronuclei of rabbit embryos

  • Peter Chrenek
  • Dusan Vasicek
  • Alexander V. Makarevich
  • Rastislav Jurcik
  • Karin Suvegova
  • Vladimir Parkanyi
  • Miroslav Bauer
  • Jan Rafay
  • Angelika Batorova
  • Rekha K. Paleyanda
Article

DOI: 10.1007/s11248-005-3238-8

Cite this article as:
Chrenek, P., Vasicek, D., Makarevich, A.V. et al. Transgenic Res (2005) 14: 417. doi:10.1007/s11248-005-3238-8

Abstract

Transgenic rabbits provide a useful biological model for the study of the regulation of mammalian genes. However, transgene integration efficiency has generally been low. Here we present a first attempt to increase the integration rate of exogenous DNA into the rabbit genome, using a double pronuclei microinjection method. Pronuclear stage rabbit embryos were recovered from superovulated NZW females, 19–20 h after hCG injection. About 5 μg/mL of exogenous DNA solution was microinjected either into one pronucleus (single microinjection, SM) or into both pronuclei (double microinjected, DM). The transgene consisted of a 2.5 kb murine whey acidic protein promoter (mWAP), 7.2 kb cDNA of the human clotting factor VIII (hFVIII), and 4.6 kb that of 3′ flanking sequences of the mWAP gene. The in vitro survival of DM embryos to the blastocyst stage was lower than that of SM embryos (68 vs. 89%). Similar results were obtained using EGFP as a control gene construct. However, there was no difference in the percentage of embryos that developed into live offspring using DM (25%) vs. SM (26%). The integration frequency of mWAP-hFVIII into the genome of transgenic rabbits was 3.3% (1/30) upon SM and 8.1% (4/49) at DM (p < 0.05). All founders transmitted the transgene to their offspring in a Mendelian fashion. The SM founder female secreted 87.4 μg/mL rhFVIII in milk, with an activity of 0.594 lU/mL. The DM founder female produced 118 μg/mL rhFVIII, with activity values of 18 lU/mL. This is the first report of transgenic rabbit production using a double microinjection technique. Our preliminary results suggest that this method can increase the efficiency of production of transgenic rabbit founders, giving a higher integration rate than single microinjection.

Keywords

EGFP Factor VIII microinjection pronucleus rabbit transgenic 

Copyright information

© Springer 2005

Authors and Affiliations

  • Peter Chrenek
    • 1
  • Dusan Vasicek
    • 1
  • Alexander V. Makarevich
    • 1
  • Rastislav Jurcik
    • 1
  • Karin Suvegova
    • 1
  • Vladimir Parkanyi
    • 1
  • Miroslav Bauer
    • 1
  • Jan Rafay
    • 1
  • Angelika Batorova
    • 2
  • Rekha K. Paleyanda
    • 3
  1. 1.Research Institute of Animal ProductionNitraSlovak Republic
  2. 2.National Hemophilia Center, Department of HematologyUniverstiy HospitalBratislavaSlovak Republic
  3. 3.American Integrated Biologics, Inc.E. WoodstockUSA