Transgenic Research

, Volume 14, Issue 6, pp 833–844

Production of Two Vaccinating Recombinant Rotavirus Proteins in the Milk of Transgenic Rabbits

Authors

    • Biologie du Développement et de la ReproductionINRA
    • Bioprotein Technologies
  • Agnès Le Saux
    • Bioprotein Technologies
  • Frédéric Guinut
    • Bioprotein Technologies
  • Bruno Passet
    • Bioprotein Technologies
  • Ruxandra Cohen
    • Bioprotein Technologies
  • Christine Merle
    • Bioprotein Technologies
  • Annie Charpilienne
    • Virologie Moléculaire & StructuraleCNRS-INRA
  • Cynthia Fourgeux
    • Virologie et Immunologie MoléculairesINRA
  • Véronique Sorel
    • Bioprotein Technologies
  • Antoine Piriou
    • Bioprotein Technologies
  • Isabelle Schwartz-Cornil
    • Virologie et Immunologie MoléculairesINRA
  • Jean Cohen
    • Virologie Moléculaire & StructuraleCNRS-INRA
  • Louis-Marie Houdebine
    • Biologie du Développement et de la ReproductionINRA
Article

DOI: 10.1007/s11248-005-1771-0

Cite this article as:
Soler, E., Saux, A.L., Guinut, F. et al. Transgenic Res (2005) 14: 833. doi:10.1007/s11248-005-1771-0

Abstract

Rotaviruses are the main cause of infantile viral gastroenteritis worldwide leading to approximately 500,000 deaths each year mostly in the developing world. For unknown reasons, live attenuated viruses used in classical vaccine strategies were shown to be responsible for intussusception (a bowel obstruction). New strategies allowing production of safe recombinant non-replicating rotavirus candidate vaccine are thus clearly needed. In this study we utilized transgenic rabbit milk as a source of rotavirus antigens. Individual transgenic rabbit lines were able to produce several hundreds of micrograms per ml of secreted recombinant VP2 and VP6 proteins in their milk. Viral proteins expressed in our model were immunogenic and were shown to induce a significant reduction in viral antigen shedding after challenge with virulent rotavirus in the adult mouse model. To our knowledge, this is the first report of transgenic mammal bioreactors allowing the rapid co-production of two recombinant viral proteins in milk to be used as a vaccine.

Keywords

bioreactormilkrotavirusVP2VP6

Abbreviations

RV

rotavirus

Tg

transgenic

; VLP

virus-like particles

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Copyright information

© Springer 2005