Transgenic Research

, Volume 13, Issue 5, pp 427–436

Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions

Authors

  • Jean-Michel Hily
    • USDA-ARS Appalachian Fruit Research Station
  • Ralph Scorza
    • USDA-ARS Appalachian Fruit Research Station
  • Tadeusz Malinowski
    • Institute of Pomology and Floriculture
  • Barbara Zawadzka
    • Institute of Pomology and Floriculture
  • Michel Ravelonandro
    • INRA-BordeauxUMR GDPP-Virologie
Article

DOI: 10.1007/s11248-004-8702-3

Cite this article as:
Hily, J., Scorza, R., Malinowski, T. et al. Transgenic Res (2004) 13: 427. doi:10.1007/s11248-004-8702-3

Abstract

Plum pox virus (PPV) is one of the most devastating diseases of Prunus species. Since few sources of resistance to PPV have been identified, transgene-based resistance offers a complementary approach to developing PPV-resistant stone fruit cultivars. C5, a transgenic clone of Prunus domestica L., containing the PPV coat protein (CP) gene, has been described as highly resistant to PPV in greenhouse tests, displaying characteristics typical of post-transcriptional gene silencing (PTGS). We show in this report that C5 trees exposed to natural aphid vectors in the field remained uninfected after 4 years while susceptible transgenic and untransformed trees developed severe symptoms within the first year. C5 trees inoculated by chip budding showed only very mild symptoms and PPV could be detected in these trees by IC-RT-PCR. The PPV-CP transgene in C5 was specifically hyper-methylated with no detectable expression. These results indicate both stability and efficiency of PTGS-based PPV resistance in plum under field conditions.

methylationPlum pox virus (PPV)post-transcriptional gene silencing (PTGS)resistancetransgenic fruit trees

Copyright information

© Kluwer Academic Publishers 2004