Transgenic Research

, Volume 13, Issue 6, pp 541–549

Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis

Authors

  • Ban Yoon Cheon
    • School of Life Sciences and BiotechnologyKorea University
  • Hae Jin Kim
    • School of Life Sciences and BiotechnologyKorea University
  • Kyung Hee Oh
    • School of Life Sciences and BiotechnologyKorea University
  • Sung Chul Bahn
    • School of Life Sciences and BiotechnologyKorea University
  • Ji Hoon Ahn
    • School of Life Sciences and BiotechnologyKorea University
  • Jang Won Choi
    • Division of Life Science, College of Natural ResourcesTaegu University
  • Sung Han Ok
    • School of Life Sciences and BiotechnologyKorea University
  • Jung Myung Bae
    • School of Life Sciences and BiotechnologyKorea University
    • School of Life Sciences and BiotechnologyKorea University
Article

DOI: 10.1007/s11248-004-2737-3

Cite this article as:
Cheon, B.Y., Kim, H.J., Oh, K.H. et al. Transgenic Res (2004) 13: 541. doi:10.1007/s11248-004-2737-3

Abstract

Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis

Keywords

Arabidopsis thalianadwarfismerythropoietin (EPO)male sterilityNicotiana tabacumoverexpressionphenotypic change

Abbreviations

cDNA

complementary DNA

CaMV

cauliflower mosaic virus

EPO

erythropoietin

PCR

polymerase chain reaction

Copyright information

© Kluwer Academic Publishers 2004