Article

Transgenic Research

, Volume 14, Issue 1, pp 41-46

Transgenic resistance to Cymbidium mosaic virus in Dendrobium expressing the viral capsid protein gene

  • Chen ChangAffiliated withDepartment of Botany, National Museum of Natural Science
  • , Ying-Chun ChenAffiliated withInstitute of Botany, Academia Sinica
  • , Yau-Heiu HsuAffiliated withGraduate Institute of Agricultural Biotechnology, National Chung Hsing University
  • , Jian-Tai WuAffiliated withInstitute of Botany, Academia Sinica
  • , Chung-Chi HuAffiliated withGraduate Institute of Agricultural Biotechnology, National Chung Hsing University
  • , Wei-Chin ChangAffiliated withInstitute of Botany, Academia Sinica
  • , Na-Sheng LinAffiliated withInstitute of Botany, Academia Sinica Email author 

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Abstract

A Taiwan isolate of Cymbidium mosaic virus (CymMV-CS) was isolated from infected Cymbidium sinesis Willd. The cDNA of the capsid protein (CP) gene was synthesized and sequenced. Alignment of this CP gene with other reported CPs revealed homologies of 92–98% at the nucleotide level and 98–99% at the amino acid level. To generate virus-resistant varieties, the CymMV-CS CP gene was transformed into Dendrobium protocorms through particle bombardment. Transformants were selected on medium supplemented with 20 mg/L hygromycin and the presence of the transgene was confirmed by polymerase chain reaction, Southern, Northern and Western blot analyses. Transgenic Dendrobium harboring the CymMV CP gene expressed a very low level of virus accumulation four months post-inoculation with CymMV, as detected by ELISA. The transgenic plants exhibited much milder symptoms than the non-transgenic plants upon challenge with CymMV virions

Keywords

capsid protein (CP) Cymbidium mosaic virus (CymMV) particle bombardment transgenic Dendrobium virus resistance