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Effects of culture medium and auxins on growth of adventitious root cultures of Cuphea aequipetala Cav. and their ability to produce antioxidant compounds

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Abstract

Cuphea aequipetala Cav. (Lythraceae), a species highly valued for its medicinal properties, is threatened in the wild. To provide an alternative source of material for production of bioactive compounds, we established adventitious root cultures of C. aequipetala and determined their phenolic compounds contents and antioxidant activity. Cultures were initiated from root tips of in vitro C. aequipetala plantlets and were grown in B5 or SH culture medium containing either indole butyric acid (IBA) or α-naphthalene acetic acid at 0, 5 or 10 µM. The maximum root biomass (1.6 g/L dry mass (DM) per L medium) was recorded after 14 days of growth in B5 + 5 µM IBA. Roots in B5 medium remained green, whereas they tended to oxidize in SH medium. The highest contents of total phenolic compounds (9.1 ± 0.1 µg gallic acid equivalents/g DM) and flavonoids (37.5 ± 0.7 µg quercetin equivalents/g DM) were in roots grown in B5 + 5 µM IBA after 14 days of growth. Root cultures accumulated mainly flavan-3-ols, whereas roots or leaves from whole plants accumulated mainly flavonols. We analyzed the antioxidant properties of root extracts using in vitro assays. Roots grown in B5 medium showed stronger free-radical scavenging activity than that of roots grown in SH medium. Our results show that adventitious root cultures of C. aequipetala are a promising system for research on antioxidant compounds biosynthesis and for scaled-up production of useful biological materials.

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Abbreviations

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

DM:

Dry mass

F&C:

Folin and Ciocalteu’s phenol reagent

GAE:

Gallic acid equivalents

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

QE:

Quercetin equivalents

TPC:

Total phenolic compounds

Trolox:

6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

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Acknowledgments

This work was supported by the Secretaría de Investigación y Posgrado del IPN-México (SIP-IPN Grant 20131756) and by Consejo Nacional de Ciencia y Tecnología (CONACyT-México) (Grant 100202). The funding bodies were not involved in the preparation or decision to submit the present paper for publication. B.P.M.B., A.R.L.L., G.T.T. and J.L.T.E. thank SIBE and EDI (IPN).

Conflicts of interest

The authors have no conflicts of interest to declare.

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Authors and Affiliations

  1. Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P. O. Box 24, 62730, Yautepec, Morelos, Mexico

    Blanca P. Martínez-Bonfil, Alma R. López-Laredo, Gabriela Trejo-Tapia & José Luis Trejo-Espino

  2. Instituto Tecnológico Superior de Álamo Temapache, Km. 6.5 Carretera Potrero del Llano-Tuxpan, Álamo Temapache, Veracruz, Mexico

    Alejandro Cruz-Hernández

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  1. Blanca P. Martínez-Bonfil
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  2. Alejandro Cruz-Hernández
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  3. Alma R. López-Laredo
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  4. Gabriela Trejo-Tapia
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  5. José Luis Trejo-Espino
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Correspondence to José Luis Trejo-Espino.

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Martínez-Bonfil, B.P., Cruz-Hernández, A., López-Laredo, A.R. et al. Effects of culture medium and auxins on growth of adventitious root cultures of Cuphea aequipetala Cav. and their ability to produce antioxidant compounds. Plant Cell Tiss Organ Cult 118, 401–408 (2014). https://doi.org/10.1007/s11240-014-0492-6

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  • DOI: https://doi.org/10.1007/s11240-014-0492-6

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