Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 113, Issue 2, pp 331–340

Expression of OsMSR3 in Arabidopsis enhances tolerance to cadmium stress

Authors

  • Yanchun Cui
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
    • University of the Chinese Academy of Sciences
  • Guoyun Xu
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
  • Manling Wang
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
  • Yan Yu
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
    • University of the Chinese Academy of Sciences
  • Mingjuan Li
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
    • University of the Chinese Academy of Sciences
  • Pedro S. C. Ferreira da Rocha
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
    • Key Laboratory for Agro-ecological Process in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of Sciences
Original Paper

DOI: 10.1007/s11240-012-0275-x

Cite this article as:
Cui, Y., Xu, G., Wang, M. et al. Plant Cell Tiss Organ Cult (2013) 113: 331. doi:10.1007/s11240-012-0275-x

Abstract

Cadmium (Cd) is a widespread heavy metal released in the environment as a result of rock mineralization and of anthropogenic activities. Cadmium is highly toxic to human health and animals, and it is urgent to remove cadmium from the environment. A multiple stress responsive gene, OsMSR3, from rice (Oryza sativa (L.)), a member of class I sHSP family, has been previously noted to be induced by cold, drought, and heat stresses. In this study, quantitative RT-PCR (qRT-PCR) analysis revealed that OsMSR3 was also induced by Cd stress. Transgenic Arabidopsis expressing OsMSR3 showed enhanced tolerance to Cd, displaying longer roots, higher survival rates and accumulated more Cd, phytochelatins (PCs), non-protein thiol (NPT) and glutathione (GSH) than wild type plants under Cd condition. Expression of OsMSR3 conferred enhanced tolerance to Cd in Arabidopsis (thaliana (L.), Heynh.) accompanied by improving expressions of bHLH transcription factors and Cd stress-related genes. Taken together, our results suggested that expression of OsMSR3 in Arabidopsis enhanced tolerance to Cd stress, and OsMSR3 may act as a positive regulator of Cd stress tolerance in plants.

Keywords

ArabidopsisOsMSR3Cadmium stressGene expressionMicroarray

Copyright information

© Springer Science+Business Media Dordrecht 2012