Original Paper

Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 113, Issue 1, pp 81-90

Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein fused to N- and C-termini of Potato virus X coat protein in Nicotiana benthamiana

  • Noemi CerovskaAffiliated withInstitute of Experimental Botany, v. v. i., Academy of Sciences of the Czech Republic
  • , Tomas MoravecAffiliated withInstitute of Experimental Botany, v. v. i., Academy of Sciences of the Czech Republic
  • , Hana HoffmeisterovaAffiliated withInstitute of Experimental Botany, v. v. i., Academy of Sciences of the Czech Republic
  • , Helena PlchovaAffiliated withInstitute of Experimental Botany, v. v. i., Academy of Sciences of the Czech Republic Email author 
  • , Helena SynkovaAffiliated withInstitute of Experimental Botany, v. v. i., Academy of Sciences of the Czech Republic
  • , Ingrid PolakovaAffiliated withDepartment of Experimental Virology, Institute of Hematology and Blood Transfusion
  • , Martina DuskovaAffiliated withDepartment of Experimental Virology, Institute of Hematology and Blood Transfusion
  • , Michal SmahelAffiliated withDepartment of Experimental Virology, Institute of Hematology and Blood Transfusion

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Abstract

Infection with Human papillomaviruses (HPVs) is linked to cervical cancer, which is one of the most common cancers found among women worldwide. Despite preventive immunization, a therapeutic vaccine, targeting infected individuals, is required. Vaccine strategies for treatment of HPV—induced cervical cancer are based on E7 and E6 oncoproteins. In this work we report the transient expression of chimeric particles containing the E6 oncoprotein from Human papillomavirus type 16 (HPV-16) in plants. We fused a mutagenized coding sequence of the E6 oncoprotein (E6GT) with the coding sequence of Potato virus X coat protein (PVX CP) both with the 5′- and 3′-terminus using linkers of different length (0, 4 or 15 amino acids). The expression in E. coli was performed to assess the characteristics of the recombinant protein prior to plant expression. The yield and immunological reactivity of the expressed proteins were screened with anti-PVX CP and E6 antibodies. The highest yields of chimeric particles were observed in the transgenic N. benthamiana expressing Potato virus A HC-Pro protein and the Tobacco mosaic virus movement protein. When inoculated on host plants, these recombinant viruses were not able to spread systemically. The obtained results revealed the new relations for design of expression cassettes for plant-based vaccine production.

Keywords

Transient expression Molecular farming Potato virus X Human papillomavirus Oncoprotein E6 Bacterial expression