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Factors influencing plant regeneration from seedling explants of Hairy nightshade (Solanum sarrachoides)

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Abstract

Hairy nightshade (Solanum sarrachoides) has the potential to be a model system for the study of plant-pathogen interactions, however, the availability of tissue culture and transformation methods would strengthen its utility. For the development of tissue culture methods, we investigated, explant type (cotyledons, hypocotyls, roots), hypocotyl explant origin, cotyledon orientation (abaxial vs. adaxial) in direct contact with the medium, gelling agents (agar and agargel) and cytokinins (zeatin and 6-benzyladenine) at different concentrations. Cotyledon explants resulted in the greatest biomass as compared to root and hypocotyl. As for hypocotyl explant origin, explants proximal to the cotyledons had a significant effect on plant regeneration. However, cotyledon orientation and gelling agent had no effect on plant regeneration. Medium supplemented with either zeatin or 6-benzyladenine at 1 mg L−1 resulted in significant shoot regeneration. Shoots rooted readily when cultured on a non-hormone based rooting medium.

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Acknowledgments

This paper was supported in part by research funds of Chonbuk National University in 2010 and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013556).

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Authors and Affiliations

  1. Department of Plant Pathology and Plant Microbe Biology, Cornell University, Ithaca, NY, 14853, USA

    Ho-Jong Ju & Stewart M. Gray

  2. The Boyce Thompson Institute for Plant Research, Inc., Ithaca, NY, 14853, USA

    Joyce Van Eck

  3. USDA/ARS, Biological Integrated Pest Management Research Unit, Ithaca, NY, 14853, USA

    Stewart M. Gray

  4. Department of Agricultural Biology, Chonbuk National University, Jeonju-city Jeonbuk, 561-756, Republic of Korea

    Ho-Jong Ju

  5. Plant Medicinal Research Center, Chonbuk National University, Jeonju-city Jeonbuk, 561-756, Republic of Korea

    Ho-Jong Ju

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  1. Ho-Jong Ju
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  2. Joyce Van Eck
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  3. Stewart M. Gray
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Correspondence to Ho-Jong Ju.

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11240_2011_20_MOESM1_ESM.ppt

Supplementary Fig. 1 Effect of gelling agent on percent of explants with shoots (A) and the number of shoots per explant (C). Effect of explant origin on percent of explants with shoots (B) and the number of shoots per explant (D). Means from 4 replicates (A and B) and from 3 replicates (C and D). Bars represent standard error (±). (PPT 174 kb)

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Ju, HJ., Van Eck, J. & Gray, S.M. Factors influencing plant regeneration from seedling explants of Hairy nightshade (Solanum sarrachoides). Plant Cell Tiss Organ Cult 108, 121–128 (2012). https://doi.org/10.1007/s11240-011-0020-x

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