Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 107, Issue 3, pp 541–552

Overexpression of TaNHX2 enhances salt tolerance of ‘composite’ and whole transgenic soybean plants

Original Paper

DOI: 10.1007/s11240-011-0005-9

Cite this article as:
Cao, D., Hou, W., Liu, W. et al. Plant Cell Tiss Organ Cult (2011) 107: 541. doi:10.1007/s11240-011-0005-9

Abstract

Salinity is a major factor resulting in extensive loss of agricultural production. Genetic transformation has become a powerful tool for studying gene function and for improving crop salt tolerance. In this study, a TaNHX2 gene was transformed into a plant cloning vector under the control of cauliflower mosaic virus 35S promoter, and then introduced into Agrobacterium rhizogenes strain K599. Explants of soybean were transformed with A. rhizogenes and ‘composite’ plants consisting of wild-type shoots and transgenic hairy roots overexpressing TaNHX2 were produced. When exposed to salt stress, ‘composite’ plants displayed high salinity tolerance at 171 mM NaCl in vermiculite and in solid medium supplemented with up to 200 mM NaCl, whereas control plants displayed chlorosis and died within 15 days under above treatment conditions. We subsequently obtained soybean plants overexpressing TaNHX2 through A. tumefaciens-mediated transformation and studied four homozygous lines of TaNHX2. Transgenic lines displayed an enhanced salt tolerance in plant biomass and flower number per plant, compared with wild type plants grown on sand culture containing 150 mM NaCl. Furthermore, transgenic plants of line C12-11 showed longer survival, less growth inhibition and greater number of flowers than wild type plants. Taken together, these results indicated that TaNHX2 gene could enhance salt tolerance of soybean, and A. rhizogenes-mediated transformation system could be used as a complementary tool of A. tumerfaciens-mediated transformation to rapidly investigate candidate gene function in soybean.

Keywords

TaNHX2 Soybean Salt tolerance Transformation Agrobacterium rhizogenes Agrobacterium tumefaciens 

Abbreviations

CaMV

Cauliflower mosaic virus

CCM

Co-cultivation medium

BA

6-Benzylaminopurine

MSB

Murashige and Skoog basal nutrient salts with B5 vitamins

RT-PCR

Reverse transcription-PCR

SD(I%)

Salt damage index (%)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbinPeople’s Republic of China
  2. 2.The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop ScienceThe Chinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  3. 3.The Graduate University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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