Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 104, Issue 1, pp 125–130

Rapid and repetitive plant regeneration of Aralia elata Seem. via somatic embryogenesis

  • Jin-Ling Dai
  • Xiao Tan
  • Ya-Guang Zhan
  • Yun-Qiang Zhang
  • Shuang Xiao
  • Ying Gao
  • Dong-Wei Xu
  • Tao Wang
  • Xiao-Chun Wang
  • Xiang-Ling You
Research Note

DOI: 10.1007/s11240-010-9801-x

Cite this article as:
Dai, JL., Tan, X., Zhan, YG. et al. Plant Cell Tiss Organ Cult (2011) 104: 125. doi:10.1007/s11240-010-9801-x

Abstract

In this work, we established a rapid and repetitive plant regeneration system for Aralia elata Seem. via primary and secondary somatic embryogenesis. Primary somatic embryogenesis was induced using leaf disks, petiole, and root segments, individually cultured for 5 weeks on Schenk and Hildebrandt (SH) (1972) medium with 0–5.0 mg/l indolebutyric acid (IBA). Our investigation demonstrated that optimal IBA concentrations of 3.0, 2.0, and 0.3 mg/l resulted in 100% somatic embryogenesis rates and averages of 11.3, 10.0, and 8.6 somatic embryos per explant for leaf disks, petiole, and root segments, respectively. The primary somatic embryos were used to conduct secondary somatic embryogenesis and the following treatments, in a gradient series, were examined: 0.3–4.0 mg/l IBA, 10–70 g/l sucrose and 0.2–3.0 mg/l abscisic acid (ABA). The results indicated that IBA was more effective than sucrose and ABA, and 3.0 mg/l IBA was the most suitable concentration for secondary somatic embryogenesis. Histological preparations indicated a multi-cellular origin of secondary somatic embryos and different morphological developmental stages during secondary somatic embryogenesis. Primary and secondary somatic embryos germinated readily and developed into normal plantlets after 2 weeks in woody plant medium (WPM, Lloyd and McCown 1980) with 20 g/l sucrose. At 4–5 cm in length, plantlets were transferred to soil (1:1 v/v of peat moss and sand) and the survival rate was 89% after 4 weeks under greenhouse conditions. This system provides a viable contribution to A. elata gene transformation, breeding and regeneration.

Keywords

Aralia elata Seem.Somatic embryogenesisPlant regenerationMedicinal plantTissue culture

Abbreviations

SH

Schenk and Hildebrandt (1972)

IBA

Indolebutyric acid

ABA

Abscisic acid

MS

Murashige and Skoog (1962)

2,4-D

2,4-Dichlorophenoxyacetic acid

BA

6-Benzyladenine

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jin-Ling Dai
    • 1
  • Xiao Tan
    • 1
  • Ya-Guang Zhan
    • 1
  • Yun-Qiang Zhang
    • 1
  • Shuang Xiao
    • 1
  • Ying Gao
    • 1
  • Dong-Wei Xu
    • 1
  • Tao Wang
    • 1
  • Xiao-Chun Wang
    • 1
  • Xiang-Ling You
    • 1
  1. 1.College of Life SciencesNortheast Forestry UniversityHarbinChina