Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 104, Issue 1, pp 1–11

Self-sterility in the mutant ‘Zigui shatian’ pummelo (Citrus grandis Osbeck) is due to abnormal post-zygotic embryo development and not self-incompatibility

  • Lijun Chai
  • Xiaoxia Ge
  • Manosh Kumar Biswas
  • Qiang Xu
  • Xiuxin Deng
Original Research Paper

DOI: 10.1007/s11240-010-9793-6

Cite this article as:
Chai, L., Ge, X., Biswas, M.K. et al. Plant Cell Tiss Organ Cult (2011) 104: 1. doi:10.1007/s11240-010-9793-6

Abstract

‘Shatian’ pummelo (Citrus grandis Osbeck), one of the main citrus cultivars in China, is self-incompatible, and its pollen tubes are believed to be arrested in style after self-pollination.We have characterized one ‘Shatian’ pummelo mutant, named ‘Zigui shatian’ pummelo. The mutant pummelo had identical DNA ploidy level, morphology (leaf shape, stoma size and density, pollen shape and size) and developmental progress of pistil and male organs to that of the common ‘Shatian’ pummelo. However, unlike the common ‘Shatian’ pummelo, ‘Zigui shatian’ is self-compatible since its pollen tubes can self-pollinate allowing for successful fertilization. Histological analyses of ‘Shatian’ pummelo further verified abnormal post-zygotic development which led to seed abortion. Simple sequence repeats (SSR) analysis revealed polymorphism in 1 of the 120 primers screened showing that ‘Zigui shatian’ and ‘Shatian’ pummelo are different at the DNA level. Taken together, these data suggested mutant ‘Zigui shatian’ pummelo might be derived from ‘Shatian’ pummelo with self-sterility by self-incongruity after self-fertilization.

Keywords

Citrus Embryonic development Self-incompatibility Natural mutant Self-sterility Pummelo 

Abbreviations

SI

Self-incompatibility

OSI

Ovarian self-incompatibility

LSI

Late-acting self-incompatibility

SSI

Sporophytic self-incompatibility

GSI

Gametophytic self-incompatibility

SSR

Simple sequence repeat

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lijun Chai
    • 1
  • Xiaoxia Ge
    • 1
  • Manosh Kumar Biswas
    • 1
  • Qiang Xu
    • 1
  • Xiuxin Deng
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

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