Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome

  • Byung-guk Kang
  • Lori Osburn
  • Dean Kopsell
  • Gerald A. Tuskan
  • Zong-Ming Cheng
Original Paper

DOI: 10.1007/s11240-009-9596-9

Cite this article as:
Kang, B., Osburn, L., Kopsell, D. et al. Plant Cell Tiss Organ Cult (2009) 99: 251. doi:10.1007/s11240-009-9596-9

Abstract

Populus serves as a model tree for biotechnology and molecular biology research due to the availability of the reference genome sequence of Populustrichocarpa (Torr. & Gray) genotype ‘Nisqually-1’. However, ‘Nisqually-1’ has been shown to be very recalcitrant to micropropagation, regeneration and transformation. In this study, a highly efficient micropropagation protocol from greenhouse-grown shoot tips of ‘Nisqually-1’ was established. The optimal micropropagation protocol involves growing in vitro shoots in plant growth regulator-free Murashige and Skoog (MS) basal medium supplemented with 3% sucrose, 0.3% Gelrite® and 5–10 g L−1 of activated charcoal. Plants grown on this medium were significantly longer, and contained significantly higher concentrations of chlorophyll. This highly effective protocol provides a consistent supply of quality leaf and stem materials throughout the year for transformation experiments and other in vitro manipulations, therefore eliminating inconsistency due to seasonal and greenhouse environmental variations and the need for repetitive tissue sterilization.

Keywords

Activated charcoal Cytokinin Gelling agent Gelrite Tissue culture Poplar 

Abbreviations

AC

Activated charcoal

BA

6-Benzyladenine

Chl a

Chlorophyll a

MS

Murashige and Skoog

PGR

Plant growth regulator

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Byung-guk Kang
    • 1
  • Lori Osburn
    • 1
  • Dean Kopsell
    • 1
  • Gerald A. Tuskan
    • 2
  • Zong-Ming Cheng
    • 1
  1. 1.Department of Plant SciencesUniversity of TennesseeKnoxvilleUSA
  2. 2.Environmental Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA

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