Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 98, Issue 1, pp 87–96

Effects of low temperature stress on rice (Oryzasativa L.) plastid ω-3 desaturase gene, OsFAD8 and its functional analysis using T-DNA mutants

  • Prakash M. Gopalakrishnan Nair
  • In-Soon Kang
  • Byoung-Yong Moon
  • Choon-Hwan Lee
Original Paper

DOI: 10.1007/s11240-009-9541-y

Cite this article as:
Gopalakrishnan Nair, P.M., Kang, IS., Moon, BY. et al. Plant Cell Tiss Organ Cult (2009) 98: 87. doi:10.1007/s11240-009-9541-y

Abstract

Expression of two rice plastidial omega-3 desaturase genes, OsFAD7 and OsFAD8 under different temperatures has been investigated. Transcript levels of OsFAD7 increased at high temperature; while those of OsFAD8 increased at low temperatures. Fatty acid analysis of homozygous OsFAD8 T-DNA knockout mutant and wild type plants have exhibited changes in fatty acid composition after cold stress, thus confirming that OsFAD8 gene codes for omega-3 fatty acid desaturase activity at low temperature. Photosynthetic efficiency and recovery of OsFAD8 knockout mutants are significantly reduced after cold stress as compared to those of wild type plants. Characterization of OsFAD8 suggests that it has a functional role in maintaining levels of trienoic fatty acids and stress tolerance at low temperatures.

Keywords

Plastid omega-3 desaturaseOsFAD8OryzasativaTemperature stressT-DNA mutantsPhotosynthetic efficiency

Abbreviations

PCR

Polymerase chain reaction

RT-PCR

Reverse transcriptase polymerase chain reaction

FAD

Fatty acid desaturase

MGDG

Monogalactosyldiacylglycerol

DGDG

Digalactosyldiacylglycerol

SQDG

Sulfoquinovosyldiacylglycerol

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

PI

Phosphatidylinositol

PC

Phosphatidylcholine

PSII

Photosystem II

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Prakash M. Gopalakrishnan Nair
    • 1
  • In-Soon Kang
    • 2
  • Byoung-Yong Moon
    • 2
  • Choon-Hwan Lee
    • 1
  1. 1.Department of Biological SciencesPusan National UniversityPusanRepublic of Korea
  2. 2.Inje UniversityGimhaeRepublic of Korea