Plant Cell, Tissue and Organ Culture (PCTOC)

, 96:307

In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets

  • Eun-A Jo
  • Rajesh Kumar Tewari
  • Eun-Joo Hahn
  • Kee-Yoeup Paek
Original Paper

DOI: 10.1007/s11240-008-9488-4

Cite this article as:
Jo, E., Tewari, R.K., Hahn, E. et al. Plant Cell Tiss Organ Cult (2009) 96: 307. doi:10.1007/s11240-008-9488-4
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Abstract

Plantlets of Alocasia amazonica were regenerated on the MS medium supplemented with different concentrations (0–9%) of sucrose. An absence of sucrose in the growth medium induced generation of leaves, however, it decreased multiplication. On contrary, sucrose supply of 6% or 9% enhanced multiplication but hampered photoautotrophic growth (generation of leaves). Increasing sucrose supply also increased sugars and starch content and number of stomata and decreased water potential and size of stomata during in vitro growth period. During ex vitro acclimatization, shoot length, root length, leaf number and root number of Alocasia plantlets grown with 3% sucrose, were found to be better among the other studied sucrose concentrations. Under ex vitro acclimatization, number of stomata, contents of various carbohydrates in the leaves were increased but size of stomata decreased with increasing sucrose supply during in vitro growth period. Moreover, water potential of leaves of plantlets, which have been grown with a sucrose concentration other than 3%, was decreased. During in vitro growth, net CO2 assimilation rate (PN), transpiration (E), stomatal conductance (gs) and variable fluorescence to maximum fluorescence ratio (Fv/Fm) were unaffected, however, during acclimatization these were changed and maximum PN, E, and gs were observed in the plantlets micropropagated with 3% sucrose. Fv/Fm was decreased severely in the plantlets micropropagated with 6% sucrose during acclimatization. Thus a sucrose concentration of 3% in the medium is appeared to be better among studied concentrations for both in vitro growth and ex vitro acclimatization of A. amazonica plantlets.

Keywords

In vitro cultureCarbohydrates concentrationStomatal developmentOsmotic stressPhotosynthesisTranspiration

Abbreviations

BA

Benzyl adenine

DAA

Days after acclimatization

E

Transpiration

Fv/Fm

Variable fluorescence to maximum fluorescence ratio

gs

Stomatal conductance

PFD

Photon flux density

PN

Net CO2 assimilation rate

RH

Relative humidity

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Eun-A Jo
    • 1
  • Rajesh Kumar Tewari
    • 1
    • 2
  • Eun-Joo Hahn
    • 1
  • Kee-Yoeup Paek
    • 1
  1. 1.Research Center for the Development of Advanced Horticultural TechnologyChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.Laboratory of Plant Nutrition, Graduate School of HorticultureChiba UniversityChibaJapan