Plant Cell, Tissue and Organ Culture

, Volume 95, Issue 2, pp 131–139

Integration of mycorrhization and photoautotrophic micropropagation in vitro: feasibility analysis for mass production of mycorrhizal transplants and inoculants of arbuscular mycorrhizal fungi


DOI: 10.1007/s11240-008-9425-6

Cite this article as:
Liu, WK. & Yang, QC. Plant Cell Tiss Organ Cult (2008) 95: 131. doi:10.1007/s11240-008-9425-6


In this paper, studies on photoautotrophic micropropagation (PM) and inoculant cultivation of arbuscular mycorrhizal fungi (AMF) were reviewed. The existing issues, which include the lack of a bio-method for improving the environmental stress resistance of plantlets in vitro or ex vitro and the mass production of AMF inoculants in commercialization for PM and inoculants cultivation, were discussed. Based on current knowledge and our analysis, we suggested that the integration of PM system and mycorrhization in vitro (here called mycorrhization-PM cultivation system) is highly feasible. Furthermore, combining mycorrhization with PM system in vitro was shown to be an efficient method for alleviating the injuries of plantlets in vitro and ex vitro caused by environmental stresses and for mass producing AMF inoculants through applying different cultivation strategies. Cultivation strategies for the mass production of mycorrhizal plants and AMF inoculants using mycorrhization-PM cultivation system were discussed.


Photoautotrophic micropropagationArbuscular mycorrhizal fungiMycorrhization-PM cultivation systemInoculantsMass production



Arbuscular mycorrhizal fungi




Carbon dioxide


Photoautotrophic micropropagation


Photosynthetic photon flux density

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Institute of Environment and Sustainable Development in Agriculture, Key Lab. for Agro-Environment & Climate Change, Ministry of AgricultureChinese Academy of Agricultural SciencesBeijingChina