Plant Cell, Tissue and Organ Culture

, Volume 93, Issue 1, pp 65–71

An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas

Original Paper

DOI: 10.1007/s11240-008-9344-6

Cite this article as:
Pathak, M.R. & Hamzah, R.Y. Plant Cell Tiss Organ Cult (2008) 93: 65. doi:10.1007/s11240-008-9344-6

Abstract

An efficient and reproducible transformation method of sonication- assisted Agrobacterium-mediated transformation (SAAT) was developed for chickpea (Cicer arietinum L.). Agrobacterium tumefaciens (LBA4404) harboring pCAMBIA1305.2 was used to transform decapitated embryo explants of two cultivars of chickpeas. By using a series of co-cultivation, callus induction, shoot initiation and root inducing media, a large number of transgenic plants were recovered. Transient expressions of GUS gene were detected by X-Gluc histochemical assay in transformed tissues. DNA analysis of T0 and T1 plants by PCR and Southern hybridization confirmed the integration of transgenes in initial and next generation transformants in different transgenic lines. The transformation efficiency was more than two times higher in SAAT treatment than simple Agrobacterium without sonication.

Keywords

Agrobacterium tumefaciens Chickpeas SAAT Transformation 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Biotechnology ProgramArabian Gulf UniversityManamaKingdom of Bahrain