Plant Cell, Tissue and Organ Culture

, Volume 92, Issue 2, pp 197–206

Agrobacterium-mediated genetic transformation of grapevine (Vitis vinifera L.) with a novel stilbene synthase gene from Chinese wild Vitis pseudoreticulata

  • Chaohong Fan
  • Ni Pu
  • Xiping Wang
  • Yuejin Wang
  • Li Fang
  • Weirong Xu
  • Jianxia Zhang
Original Paper

DOI: 10.1007/s11240-007-9324-2

Cite this article as:
Fan, C., Pu, N., Wang, X. et al. Plant Cell Tiss Organ Cult (2008) 92: 197. doi:10.1007/s11240-007-9324-2

Abstract

A novel stilbene synthase gene (STS), cloned from Chinese wild Vitis pseudoreticulata (W. T. Wang) and responsible for synthesis of the phytoalexin resveratrol in grapevine, was successfully transferred into V. vinifera L. cv. Thompson Seedless via Agrobacterium tumefaciens-mediated transformation. Using transformation procedures developed in the present study, 72% GFP-positive germinated embryos were produced with about 38% of transformed embryos regenerated into normal plantlets. Integration of the STS gene into the transgenic plants was verified by PCR and Southern blot analysis. Expression of the STS gene was detected by high performance liquid chromatography (HPLC), which showed that the resveratrol concentration in the transgenic plants was 5.5 times higher than that in non-transformed control plants.

Keywords

Genetic transformationGrapevineResveratrolSomatic embryogenesisStilbene synthase geneVitis

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

BAP

6-Benzylaminopurine

CTAB

Cetyltrimethylammonium bromide

GFP

Green fluorescent protein

GUS

β-Glucuronidase

Hyg

Hygromycin B

HPLC

High-performance liquid chromatography

HPT II

Hygromycin phosphotransferase

NOA

2-Naphthoxyacetic acid

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Chaohong Fan
    • 1
    • 2
    • 3
  • Ni Pu
    • 1
    • 2
    • 3
  • Xiping Wang
    • 1
    • 2
    • 3
  • Yuejin Wang
    • 1
    • 2
    • 3
  • Li Fang
    • 1
    • 2
    • 3
  • Weirong Xu
    • 1
    • 2
    • 3
  • Jianxia Zhang
    • 1
    • 2
    • 3
  1. 1.Key Laboratory of Horticultural Plant Germplasm Resources & Genetic Improvement in Northwest ChinaMinistry of Agriculture of ChinaYanglingP. R. China
  2. 2.Key Laboratory of Molecular Biology of AgricultureYanglingP. R. China
  3. 3.College of Horticulture Northwest A & F UniversityYanglingP. R. China