Plant Cell, Tissue and Organ Culture

, Volume 92, Issue 2, pp 233–238

Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

  • Emilio Mendoza-de Gyves
  • Farida Rosana Mira
  • Fabrizio Ruiu
  • Eddo Rugini
Research note

DOI: 10.1007/s11240-007-9314-4

Cite this article as:
Mendoza-de Gyves, E., Rosana Mira, F., Ruiu, F. et al. Plant Cell Tiss Organ Cult (2008) 92: 233. doi:10.1007/s11240-007-9314-4

Abstract

Dikegulac, a growth regulator, was shown to stimulate in vitro shoot multiplication of olive cultivars Canino, Frantoio, Moraiolo, but not cultivars Rosciola and Piantone di Moiano. The Rugini Olive Medium including zeatin (4.5 μM) was used supplemented with a range of dikegulac concentrations (0–133.4 μM). An optimal result in number of shoots and nodes was obtained on cultivars Canino, Frantoio and Moraiolo at 66.7 μM dikegulac. Higher concentrations did not stimulate additional shoot and node formation and resulted in a drastic reduction in height of shoots. Elongated shoots were rooted and acclimatised and showed normal development compared to control plants.

Keywords

Apical dominance Axillary shoots Dikegulac Growth regulators Tissue culture In vitro propagation Shoot proliferation Zeatin 

Abbreviations

IBA

Indole-3-butyric acid

BAP

Benzyl amino purine

TDZ

Thidiazuron

TIBA

Tri-iodo benzoic acid

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Emilio Mendoza-de Gyves
    • 1
  • Farida Rosana Mira
    • 2
  • Fabrizio Ruiu
    • 1
  • Eddo Rugini
    • 1
  1. 1.Dipartimento Produzione VegetaleUniversità degli Studi della TusciaViterboItaly
  2. 2.Biotech Center, Agency for The Assessment and Application of TechnologySerpongIndonesia

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