Plant Cell, Tissue and Organ Culture

, Volume 86, Issue 3, pp 349–358

Salt and paraquat stress tolerance results from co-expression of the Suaeda salsa glutathione S-transferase and catalase in transgenic rice

Original paper

DOI: 10.1007/s11240-006-9133-z

Cite this article as:
Zhao, F. & Zhang, H. Plant Cell Tiss Organ Cult (2006) 86: 349. doi:10.1007/s11240-006-9133-z

Abstract

GST (Glutathione S-transferase, EC 2.5.1.18) and CAT (Catalase, EC 1.11.1.6) play important roles in oxidative stress resistance. In this study, we transferred both GST and CAT1 of Suaeda salsa into rice (Oryza sativa cv. Zhonghua No.11) by Agrobacterium tumefaciens-mediated transformation under the control of cauliflower mosaic virus (CaMV) 35S promoter, and investigated whether co-expressing the GST and CAT1 in transgenic rice could reduce oxidative damage. Salt and paraquat stresses were applied. The data showed that co-expression of the GST and CAT1 resulted in greater increase of CAT and SOD (Superoxide Dismutase, EC 1.15.1.1) activity in the transgenics compared to non-transgenics following both stress imposition. Whereas the significant increase of GST activity in transgenics only occurred in paraquat stressed plants. While the generation of H2O2, Malon dialdehyde and plasma membrane relative electrolyte leakage decreased in the transgenics than in non-transgenics under the same conditions. Moreover, the transgenic rice seedlings showed markedly enhanced tolerance to salt stress compared with non-transgenics upon 200 mM NaCl treatment in greenhouse. The enhancement of the active oxygen-scavenging system that led to increased oxidative stress protection in GST  + CAT1-transgenic rice plants could result not only from increased GST and CAT activity but also from the combined increase in SOD activity.

Keywords

Antioxidant geneCATGSTH2O2Malon dialdehydeOxidative stressPlasma membrane relative electrolyte leakage

Abbreviations

CDNB-1

Chloro-2,4-dinitrobenzene

DAB-3

3-Diaminobenzidine

MV

Methyl viologen

PEL

Plasma membrane relative electrolyte leakage

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Life Science CollegeShandong University of TechnologyZiboP.R. China
  2. 2.Key Laboratory of Plant Stress, Life Science CollegeShandong Normal UniversityJinanP.R. China