, Volume 82, Issue 3, pp 349-356

The heritable changes in metabolic profiles of plants regenerated in different types of in vitro culture

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Abstract

In this work we show how three types of cucumber in vitro cultures – leaf callus culture, cytokinin dependent cell suspension and liquid culture of meristematic clumps – influence the metabolite profiles of plants in the first generative progeny. Based on this study we conclude that there exists a specific and inheritable metabolic fingerprint reflecting the history of previous generations, probably related to specific stress factors accompanying the passage through different types of culture. The leaf callus culture generated the highest heritable differences in metabolite content and was the most distinctly separated cluster in PCA analysis. The smallest number of variable metabolites characterizes the plants regenerated from cytokinin dependent cell suspension whereas the liquid culture of meristematic clumps induced slightly more changes. Changes induced by these two culture types were not as pronounced as in the case of leaf callus culture. However the plants after these types of culture were well separated from the control on PCA diagram. The highest changes were over 2-fold increases in cystin and galactose-6-P and over 2-fold decreases in aspartate, myo-inositol, hydroxylamine, phosphate and putrescine. These changes concerned the plants, which were one generation after the leaf callus culture. The possible nature of observed heritable changes is discussed.