Plant Cell, Tissue and Organ Culture

, Volume 80, Issue 1, pp 59–67

Selection in vitro and accumulation of phytochelatins in cadmium tolerant cell line of cucumber (Cucumis sativus)

Article

DOI: 10.1007/s11240-004-8808-6

Cite this article as:
Gzyl, J. & Gwóźdź, E.A. Plant Cell Tiss Organ Cult (2005) 80: 59. doi:10.1007/s11240-004-8808-6

Abstract

Cucumber (Cucumis sativus L.) cells from suspension culture were selected for their ability to grow and divide rapidly in toxic concentration of cadmium. As a result of selection a cell suspension tolerant to 100 μM cadmium chloride (CdCl2) was initiated. The selected tolerant line exhibited stable and repeatable increase in fresh and dry weight of cells in the presence of cadmium. The accumulated level of phytochelatins in cadmium sensitive (unselected) and tolerant cell line was measured by high performance liquid chromatography (HPLC) after 3, 24 h and 5 days of cadmium treatment. It was shown that in both cell lines Cd induced accumulation of phytochelatins and simultaneous glutathione depletion occurred. No distinct changes were found after 3 and 24 h of cadmium treatment whereas after 5 days of exposure to the metal, the level of phytochelatins was two times higher in the sensitive cell line as compared to the tolerant one. The accumulation of phytochelatins was correlated with cadmium concentration that increased in both cell lines during the course of cell exposure to metal. However, the level of cadmium was always lower in the tolerant cell line. The results showed no direct correlation between the tolerance of cucumber cells to Cd and the accumulated level of phytochelatins. Other mechanisms responsible for the increased tolerance of cucumber cells exposed to Cd are discussed.

Keywords

cadmium accumulation cell suspension glutathione metal tolerance 

Abbreviations:

BSO

buthionine sulfoximine

GSH

glutathione

HMW

high molecular weight

LMW

low molecular weight

PCs

phytochelatins

SH

thiol

SL

sensitive line

TL

tolerant line

TTC

triphenyl tetrazolium chloride

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Laboratory of Plant EcophysiologyAdam Mickiewicz UniversityPoznańPoland