Journal of Thrombosis and Thrombolysis

, Volume 25, Issue 3, pp 280–283

Prevalence of factor V Leiden (G1691A) and prothrombin (G20210A) among Kurdish population from Western Iran

  • Zohreh Rahimi
  • Asad Vaisi-Raygani
  • Hadi Mozafari
  • Hadi Kharrazi
  • Mansour Rezaei
  • Ronald L. Nagel
Article

DOI: 10.1007/s11239-007-0052-6

Cite this article as:
Rahimi, Z., Vaisi-Raygani, A., Mozafari, H. et al. J Thromb Thrombolysis (2008) 25: 280. doi:10.1007/s11239-007-0052-6

Abstract

Background

The mutation in factor V (FV) G1691A, known as factor V Leiden, and prothrombin (FII) gene G20210A are the two most prevalent causes of inherited thrombophilia. The present study reports the prevalence of factor V Leiden and the prothrombin G20210A gene mutations among healthy individuals of Kurdish ethnic background in Western Iran.

Methods

Four hundred thirty-four healthy unrelated individuals, 255 male and 179 female, with a mean age of 28.7 ± 15.5 from the Kermanshah Province of Iran were studied for prothrombin G20210A mutation. The factor V Leiden mutation was studied in 404 healthy individuals, of whom 232 were male and 172 were female. The factor V Leiden and prothrombin G20210A were detected by polymerase chain reaction–restriction fragment-length polymorphism (PCR-RFLP) method using Mnl I and Hind III restriction enzymes, respectively.

Results

Among 434 individuals studied for prothrombin G20210A mutation seven carried this mutation as heterozygous (four female subjects and three male), giving a prevalence of 1.6% [95% confidence intervals (CI) 0.5–2.7) and an allele frequency of 0.8%. No homozygous prothrombin 20210AA was found. Factor V G1691A mutation was detected as heterozygous in 11 of 404 healthy individuals (five female and six male) and as homozygous in one male indicating a prevalence of 2.97% (95% CI 1.3–4.6) and allele frequency of 1.6%.

Conclusions

Our results indicated that the factor V Leiden and prothrombin G20210A mutations are not rare among populations of Western Iran and that the relationship between venous thrombophilia and these mutations have to be further studied in Western Iran population, which, in turn, may suggest a causal effect.

Keywords

Factor V LeidenPrevalenceProthrombin G20210AWestern Iran

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Zohreh Rahimi
    • 1
    • 2
  • Asad Vaisi-Raygani
    • 2
  • Hadi Mozafari
    • 1
  • Hadi Kharrazi
    • 2
  • Mansour Rezaei
    • 3
  • Ronald L. Nagel
    • 4
    • 5
  1. 1.Medical Biology Research CentreKermanshah University of Medical SciencesKermanshahIran
  2. 2.Department of Biochemistry, Medical SchoolKermanshah University of Medical SciencesKermanshahIran
  3. 3.Department of Biostatistics, Medical SchoolKermanshah University of Medical SciencesKermanshahIran
  4. 4.Department of Medicine, Division of HematologyAlbert Einstein College of MedicineBronxUSA
  5. 5.Department of Physiology and BiophysicsAlbert Einstein College of MedicineBronxUSA