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Diplostomum von Nordmann, 1832 (Digenea: Diplostomidae) in the sub-Arctic: descriptions of the larval stages of six species discovered recently in Iceland

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Abstract

Frequent infections with Diplostomum spp. (Digenea: Diplostomidae) were found in the freshwater snail Radix peregra (Müller) and three fish species, the salmonids Salmo trutta fario L., Salvelinus alpinus (L.) and the gasterosteid Gasterosteus aculeatus L., collected in four lakes in south-western Iceland in 2012. Detailed analysis of the isolates integrating molecular, morphological and ecological data revealed that these belong to Diplostomum spathaceum (Rudolphi, 1819) and five putative new species (three infecting both snails and fish). This paper provides detailed descriptions of the metacercariae of the six species-level lineages of Diplostomum spp. and of the cercariae of three of the lineages discovered in Iceland with comments on the application of ITS1 rDNA for species distinction within Diplostomum von Nordmann, 1832 in the light of the novel data. We strongly suggest the use of molecular evidence based on cox1 gene sequences (in addition to ITS1-5.8S-ITS2 sequences) in association with detailed assessment of the morphology of the larval stages in future studies of Diplostomum spp. in fish and snails.

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Acknowledgements

This study was supported by the Czech Science Foundation (AF, AK, SG, TS, grant P505/12/G112), Institute of Parasitology (AF, AK, SG, TS, RVO: 60077344) and the University of Iceland (KS, Research Fund of the University of Iceland).

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Authors and Affiliations

  1. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Anna Faltýnková, Simona Georgieva, Aneta Kostadinova, Isabel Blasco-Costa & Tomáš Scholz

  2. Faculty of Science, University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Simona Georgieva

  3. Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand

    Isabel Blasco-Costa

  4. Laboratory of Parasitology, Institute for Experimental Pathology, University of Iceland, Keldur, 112, Reykjavík, Iceland

    Karl Skírnisson

Authors
  1. Anna Faltýnková
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  2. Simona Georgieva
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  3. Aneta Kostadinova
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  4. Isabel Blasco-Costa
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  5. Tomáš Scholz
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  6. Karl Skírnisson
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Correspondence to Anna Faltýnková.

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Faltýnková, A., Georgieva, S., Kostadinova, A. et al. Diplostomum von Nordmann, 1832 (Digenea: Diplostomidae) in the sub-Arctic: descriptions of the larval stages of six species discovered recently in Iceland. Syst Parasitol 89, 195–213 (2014). https://doi.org/10.1007/s11230-014-9517-0

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  • DOI: https://doi.org/10.1007/s11230-014-9517-0

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