Abstract
A new entomopathogenic nematode, Steinernema boemarei n. sp., is described from southern France. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. Both molecular and morphological data indicate that the new species belongs to the ‘glaseri-group’ of Steinernema spp. Key morphological diagnostic traits for S. boemarei n. sp. include the presence of prominent deirids (cervical papillae) on adult males, the morphology of the spicules and gubernaculum, and the arrangement of the 23 genital papillae of the first generation males. Additionally, morphometric traits of the third-stage infective juvenile, including total body length (mean 1,103 μm), tail length (mean 86 μm), location of the excretory pore (mean 91 μm), and D% (mean 63), E% (mean 106) and H% (mean 41) values are definitive. In addition to these morphological characters, analysis of both 28S and ITS rDNA sequences depict this Steinernema species as a distinct and unique entity.
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Acknowledgements
We are thankful to Y. Flores-Lara for assistance with the SEM images. The authors also express their gratitude to San Miguel High School, Tucson Arizona, and the Undergraduate Biology Research Program (UBRP), University of Arizona, who funded M. Skeie during her summer internship in the laboratory of S.P.S. This research was funded in part by a National Science Foundation grant (NSF-DEB award no. 060899) to S.P. Stock.
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Lee, MM., Sicard, M., Skeie, M. et al. Steinernema boemarei n. sp. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern France. Syst Parasitol 72, 127–141 (2009). https://doi.org/10.1007/s11230-008-9166-2
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DOI: https://doi.org/10.1007/s11230-008-9166-2