, Volume 57, Issue 5, pp 1033-1041
Date: 20 Mar 2009

Creation of biocatalysts with prescribed properties

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Abstract

Oxidoreductases are widely used in different branches of industry (particularly, in pharmaceutical) and analytical biotechnology. To optimize the use and to reduce production cost of a target product, the properties of a biocatalyst should be modified to meet requirements of a specific process or method of analysis. Problem of creation of biocatalysts with prescribed properties includes complex set of R&D such as an engineering of enzyme properties, construction of recombinant strains overproducing the target protein, development of a large-scale downstream process and preparation of a working form of a biocatalysts. In the present work, the basic features and practical examples of such an over-all approach are considered for formate dehydrogenase and D-amino acid oxidase.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1014–1022, May, 2008.