, Volume 57, Issue 5, pp 1033-1041
Date: 20 Mar 2009

Creation of biocatalysts with prescribed properties

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


Oxidoreductases are widely used in different branches of industry (particularly, in pharmaceutical) and analytical biotechnology. To optimize the use and to reduce production cost of a target product, the properties of a biocatalyst should be modified to meet requirements of a specific process or method of analysis. Problem of creation of biocatalysts with prescribed properties includes complex set of R&D such as an engineering of enzyme properties, construction of recombinant strains overproducing the target protein, development of a large-scale downstream process and preparation of a working form of a biocatalysts. In the present work, the basic features and practical examples of such an over-all approach are considered for formate dehydrogenase and D-amino acid oxidase.

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1014–1022, May, 2008.