Review

Russian Chemical Bulletin

, Volume 57, Issue 5, pp 1033-1041

First online:

Creation of biocatalysts with prescribed properties

  • V. I. TishkovAffiliated withDepartment of Chemistry, M. V. Lomonosov Moscow State UniversityA. N. Bach Institute of Biochemistry, Russian Academy of Sciences Email author 
  • , S. S. SavinAffiliated withDepartment of Chemistry, M. V. Lomonosov Moscow State UniversityA. N. Bach Institute of Biochemistry, Russian Academy of Sciences
  • , S. V. KhoronenkovaAffiliated withDepartment of Chemistry, M. V. Lomonosov Moscow State UniversityInnovations and High Technologies MSU Ltd.

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Abstract

Oxidoreductases are widely used in different branches of industry (particularly, in pharmaceutical) and analytical biotechnology. To optimize the use and to reduce production cost of a target product, the properties of a biocatalyst should be modified to meet requirements of a specific process or method of analysis. Problem of creation of biocatalysts with prescribed properties includes complex set of R&D such as an engineering of enzyme properties, construction of recombinant strains overproducing the target protein, development of a large-scale downstream process and preparation of a working form of a biocatalysts. In the present work, the basic features and practical examples of such an over-all approach are considered for formate dehydrogenase and D-amino acid oxidase.

Key words

oxidoreductases formate dehydrogenase d-amino acid oxidase genetic and protein engineering recombinant strains chiral synthesis coenzymes recovery