Creation of biocatalysts with prescribed properties
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- Tishkov, V.I., Savin, S.S. & Khoronenkova, S.V. Russ Chem Bull (2008) 57: 1033. doi:10.1007/s11172-008-0132-6
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Oxidoreductases are widely used in different branches of industry (particularly, in pharmaceutical) and analytical biotechnology. To optimize the use and to reduce production cost of a target product, the properties of a biocatalyst should be modified to meet requirements of a specific process or method of analysis. Problem of creation of biocatalysts with prescribed properties includes complex set of R&D such as an engineering of enzyme properties, construction of recombinant strains overproducing the target protein, development of a large-scale downstream process and preparation of a working form of a biocatalysts. In the present work, the basic features and practical examples of such an over-all approach are considered for formate dehydrogenase and D-amino acid oxidase.