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Regular Consumption of an Antioxidant-rich Juice Improves Oxidative Status and Causes Metabolome Changes in Healthy Adults

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Abstract

An improvement in oxidative status is associated with a reduction in the incidence of several chronic diseases. However, daily intake of antioxidants in Western diets is decreasing. This study evaluates the effect of daily consumption of an antioxidant-rich juice (ARJ) on oxidative status, cardiovascular disease risk parameters, and untargeted plasma and urine metabolomes. Twenty-eight healthy young adults participated in an 8-week clinical trial by drinking 200 mL of ARJ (pomegranate and grape) daily. At the end of the study, the subjects showed a significant decrease (−29 %) in plasma lipid oxidation (malondialdehyde concentration), and a significant increase (+115 %) in plasma antioxidant capacity. Plasma and urine metabolomes were also significantly modified and some ions modified in urine were identified, including metabolites of polyphenols, ascorbic acid and biliary acids. No significant changes were observed in lipid profile, inflammation, blood pressure or glycaemia. These results show that incorporating antioxidant-rich beverages into common diets may improve oxidative status in healthy subjects.

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Abbreviations

ABTS:

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

AC:

Antioxidant capacity

ALP:

Alkaline phosphatase

ALT/GPT:

Alanine aminotransferase/glutamic pyruvic transaminase

ARJ:

Antioxidant-rich juice

AST/GOT:

Aspartate aminotransferase/glutamic oxaloacetic transaminase

CVD:

Cardiovascular disease

FRAP:

Ferric reducing-antioxidant power

LC/MS:

Liquid chromatography/mass spectrometry

MDA:

Malondialdehyde

PCA:

Principal component analysis

Q-TOF:

Quadrupole time of flight

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Acknowledgments

We thank M.L. García-González for technical assistance.

Funding

This research was supported by the Spanish Ministry of Science and Innovation (AGL2011-27741). M.E. D-R. and J. P-J. are recipients of JAE-Doc and Juan de la Cierva postdoctoral contracts from the CSIC and the Spanish Ministry of Science and Innovation, respectively.

Conflict of Interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Jose Antonio Novais, 10, 28040, Madrid, Spain

    M. Elena Díaz-Rubio, Jara Pérez-Jiménez & Fulgencio Saura-Calixto

  2. Unit Service of Analitycal Techniques, Instrumentation and Microbiology (USTA), Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Jose Antonio Novais, 10, 28040, Madrid, Spain

    Miguel Ángel Martínez-Bartolomé & Inmaculada Álvarez

Authors
  1. M. Elena Díaz-Rubio
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  2. Jara Pérez-Jiménez
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  3. Miguel Ángel Martínez-Bartolomé
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  4. Inmaculada Álvarez
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  5. Fulgencio Saura-Calixto
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Corresponding author

Correspondence to Fulgencio Saura-Calixto.

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Díaz-Rubio, M.E., Pérez-Jiménez, J., Martínez-Bartolomé, M.Á. et al. Regular Consumption of an Antioxidant-rich Juice Improves Oxidative Status and Causes Metabolome Changes in Healthy Adults. Plant Foods Hum Nutr 70, 9–14 (2015). https://doi.org/10.1007/s11130-014-0455-4

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  • DOI: https://doi.org/10.1007/s11130-014-0455-4

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