Plant Foods for Human Nutrition

, Volume 67, Issue 4, pp 384–392

Chemical Composition and Anti-inflammatory Properties of the Unsaponifiable Fraction from Awara (Astrocaryum vulgare M.) Pulp Oil in Activated J774 Macrophages and in a Mice Model of Endotoxic Shock

Authors

  • Emilie Bony
    • Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de PharmacieUniversité Montpellier I
    • Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYSTUMR Qualisud
  • Frédéric Boudard
    • Laboratoire d’Immunologie, Faculté de PharmacieUniversité Montpellier I
  • Emilie Dussossoy
    • Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de PharmacieUniversité Montpellier I
  • Karine Portet
    • Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de PharmacieUniversité Montpellier I
  • Pierre Brat
    • Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYSTUMR Qualisud
  • Jean Giaimis
    • Laboratoire d’Immunologie, Faculté de PharmacieUniversité Montpellier I
    • Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de PharmacieUniversité Montpellier I
Original Paper

DOI: 10.1007/s11130-012-0323-z

Cite this article as:
Bony, E., Boudard, F., Dussossoy, E. et al. Plant Foods Hum Nutr (2012) 67: 384. doi:10.1007/s11130-012-0323-z

Abstract

Awara (Astrocaryum vulgare M.) pulp oil has been shown to possess anti-inflammatory properties in vivo, and contains an unsaponifiable matter rich in bioactive compounds. This study focused on the ethanolic unsaponifiable fraction (EUF) of awara pulp oil. Its chemical composition has been characterized: carotenoid, phytosterol, and tocopherol contents represent 125.7, 152.6, and 6.8 μg/mg of EUF, respectively. We further evaluated this fraction for anti-inflammatory properties in J774 macrophages activated by lipopolysaccharide (LPS) plus interferon (IFN) γ to understand the biological effects of awara pulp oil. EUF strongly decreased nitric oxide (NO), prostaglandin E2, tumour necrosis factor (TNF) α, and interleukin (IL) -6 and -10 production in activated J774 cells. Moreover, it inhibited expression of inducible NO synthase and cyclooxygenases-2 in vitro. The anti-inflammatory properties of EUF were also confirmed in vivo by modulation of TNFα, IL-6 and IL-10 serum concentration in an endotoxic shock model. Pre-treatment with awara oil fraction offers promise as a protective means to lower the production of excessive amounts of pro-inflammatory molecules.

Keywords

Awara (Astrocaryum vulgare M.) Unsaponifiable fraction Carotenoids Phytosterols Tocopherols Anti-inflammatory J774 macrophages Endotoxic shock

Abbreviations

COX

Cyclooxygenases

EUF

Ethanolic unsaponifiable fraction

IFN

Interferon

IL

Interleukin

LPS

Lipopolysaccharide

NOS

Nitric oxide synthase

ORAC

Oxygen radical absorbance capacity

PG

Prostaglandin

ROS

Reactive oxygen species

SNP

Sodium nitroprusside

TNF

Tumour necrosis factor

Supplementary material

11130_2012_323_MOESM1_ESM.doc (160 kb)
ESM 1 (DOC 159 kb)

Copyright information

© Springer Science+Business Media New York 2012