Plant Foods for Human Nutrition

, Volume 65, Issue 3, pp 193–199

Dietary Broccoli Sprouts Protect Against Myocardial Oxidative Damage and Cell Death During Ischemia-Reperfusion

ORIGINAL PAPER

DOI: 10.1007/s11130-010-0182-4

Cite this article as:
Akhlaghi, M. & Bandy, B. Plant Foods Hum Nutr (2010) 65: 193. doi:10.1007/s11130-010-0182-4

Abstract

Cruciferous vegetables are known for antioxidant and anti-carcinogenic effects. In the current study we asked whether dietary broccoli sprouts can protect the heart from ischemia-reperfusion. Rats were fed either control diet (sham and control groups) or a diet mixed with 2% dried broccoli sprouts for 10 days. After 10 days the isolated hearts were subjected to ischemia for 20 min and reperfusion for 2 h, and evaluated for cell death, oxidative damage, and Nrf2-regulated phase 2 enzyme activities. Broccoli sprouts feeding inhibited markers of necrosis (lactate dehydrogenase release) and apoptosis (caspase-3 activity) by 78–86%, and decreased indices of oxidative stress (thiobarbituric acid reactive substances and aconitase inactivation) by 82–116%. While broccoli sprouts increased total glutathione and activities of the phase 2 enzymes glutamate cysteine ligase and quinone reductase in liver, they did not affect these in ischemic-reperfused heart. While the mechanism is not clear, the results show that a relatively short dietary treatment with broccoli sprouts can strongly protect the heart against oxidative stress and cell death caused by ischemia-reperfusion.

Keywords

Antioxidant phytochemicals Broccoli sprouts Dietary treatment Ischemia-reperfusion Phase 2 enzymes Rat heart 

Abbreviations

DCIP

2,6-dichloroindophenol

GCL

glutamate cysteine ligase

GSH

reduced glutathione

GSSG

oxidized glutathione

IR

ischemia-reperfusion

LDH

lactate dehydrogenase

PMSF

phenylmethylsulphonyl fluoride

QR

quinone reductase

TBARS

thiobarbituric acid reactive substances

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.College of Health and NutritionShiraz University of Medical SciencesShirazIran
  2. 2.College of Pharmacy and NutritionUniversity of SaskatchewanSaskatoonCanada

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