Plant Foods for Human Nutrition

, 64:244

Antioxidative and Antigenotoxic Effects of Garlic (Allium sativum L.) Prepared by Different Processing Methods

Authors

  • Jae-Hee Park
    • Department of Food and NutritionKyungnam University
  • Yoo Kyoung Park
    • Department of Medical NutritionKyunghee University
    • Department of Food and NutritionKyungnam University
Original Paper

DOI: 10.1007/s11130-009-0132-1

Cite this article as:
Park, J., Park, Y.K. & Park, E. Plant Foods Hum Nutr (2009) 64: 244. doi:10.1007/s11130-009-0132-1

Abstract

This study describes the antioxidant activities and antigenotoxic effects of garlic extracts prepared by different processing methods. Aged-garlic extract (AGE) showed a significantly higher total phenolic content (562.6 ± 1.92 mg/100 g garlic acid equivalents) than those of raw garlic extract (RGE) or heated garlic extract (HGE). The SC50 for DPPH RSA in HGE was significantly the highest at 2.1 mg/ml. The SC50 for SOD-like activity in garlic extracts was, in decreasing order, RGE (7.3 mg/ml) > AGE (8.5 mg/ml) > HGE (9.2 mg /ml). The ED50 of AGE was the highest (19.3 μg/ml) regarding H2O2 induced DNA damage and its inhibition rate was 70.8%. The ED50 of RGE for 4-hydroxynonenal (a lipid peroxidation product) induced DNA damage was 38.6 μg/ml, followed by AGE > HGE. Although the heat treatment of garlic tended to decrease the TPC and SOD-like activity and increased DPPH RSA, garlic, in general, has significant antioxidant activity and protective effects against oxidative DNA damage regardless of processing method.

Keywords

Aged-garlicTotal polyphenolDPPHSOD-like activityAntigenotoxic effect

Abbreviations

RGE

Raw garlic extract

HGE

Heated garlic extract

AGE

Aged garlic extract

ED50

The estimated dose for 50% reduction in oxidative DNA damage

SC50

The concentration required for scavenging 50% of activity

DPPH

2,2-Diphenyl-1-picrylhydrazyl

DMSO

Dimethyl sulfoxide

TPC

Total phenolic contents

RSA

Radical scavenging activity

SOD

Superoxide dismutase

FCR

Folin–Ciocalteu reagent

HNE

4-hydroxynonenal

Copyright information

© Springer Science+Business Media, LLC 2009