Photosynthesis Research

, Volume 98, Issue 1, pp 523–527

Analysis of xenon binding to photosystem II by X-ray crystallography

  • J. W. Murray
  • K. Maghlaoui
  • J. Kargul
  • M. Sugiura
  • J. Barber
Regular paper

DOI: 10.1007/s11120-008-9366-2

Cite this article as:
Murray, J.W., Maghlaoui, K., Kargul, J. et al. Photosynth Res (2008) 98: 523. doi:10.1007/s11120-008-9366-2

Abstract

In order to investigate oxygen binding and hydrophobic cavities in photosystem II (PSII), we have introduced xenon under pressure into crystals of PSII isolated from Thermosynechococcus elongatus and used X-ray anomalous diffraction analyses to identify the xenon sites in the complex. Under the conditions employed, 25 Xe-binding sites were identified in each monomer of the dimeric PSII complex. The majority of these were distributed within the membrane spanning portion of the complex with no obvious correlation with the previously proposed oxygen channels. One binding site was located close to the haem of cytochrome b559 in a position analogous to a Xe-binding site of myoglobin. The only Xe-binding site not associated with the intrinsic subunits of PSII was within the hydrophobic core of the PsbO protein.

Keywords

Photosystem IIXenon-bindingX-ray crystallographyOxygen channelCytochrome b559PsbO protein

Abbreviations

CP43

Chlorophyll-binding PsbC protein

D1

Reaction centre PsbA protein

PSII

Photosystem II

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • J. W. Murray
    • 1
  • K. Maghlaoui
    • 1
  • J. Kargul
    • 1
  • M. Sugiura
    • 2
  • J. Barber
    • 1
  1. 1.Division of Molecular BiosciencesImperial College LondonLondonUK
  2. 2.Cell-Free Science and Technology Research CenterEhime UniversityMatsuyamaJapan