Photosynthesis Research

, Volume 95, Issue 2, pp 191–196

Chlorosome lipids from Chlorobium tepidum: characterization and quantification of polar lipids and wax esters

  • Peder Grove Sørensen
  • Raymond P. Cox
  • Mette Miller
Regular Paper

DOI: 10.1007/s11120-007-9242-5

Cite this article as:
Sørensen, P.G., Cox, R.P. & Miller, M. Photosynth Res (2008) 95: 191. doi:10.1007/s11120-007-9242-5

Abstract

We have extracted polar lipids and waxes from isolated chlorosomes from the green sulfur bacterium Chlorobium tepidum and determined the fatty acid composition of each lipid class. Polar lipids amounted to 4.8 mol per 100 mol bacteriochlorophyll in the chlorosomes, while non-polar lipids (waxes) were present at a ratio of 5.9 mol per 100 mol bacteriochlorophyll. Glycolipids constitute 60 % of the polar lipids while phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and an aminoglycosphingolipid make up respectively 15, 3, 8 and 12 %. A novel glycolipid was identified as a rhamnose derivative of monogalactosyldiacylglycerol, while the other major glycolipid was monogalactosyldiacylglycerol. Tetradecanoic acid was the major fatty acid in the aminoglycosphingolipid, while the other polar lipids contained predominantly hexandecanoic acid. The chlorosome waxes are esters of unbranched fatty acids and fatty alcohols with 14 or 16 carbon atoms, joined to form molecules with between 28 and 32 carbon atoms. The stoichiometry between lipids and bacteriochlorophyll suggests that much of the chlorosome surface is covered by protein.

Keywords

AminoglycosphingolipidGlycolipidGreen sulfur bacteriaLight-harvesting antenna

Abbreviations

AGS

Aminoglycosphingolipid

BChl

Bacteriochlorophyll

DPG

Diphosphatidylglycerol

FAME

Fatty acid methyl ester

MGDG

Monogalactosyldiacylglycerol

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

RGDG

Rhamnosylgalactosyldiacylglycerol

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Peder Grove Sørensen
    • 1
  • Raymond P. Cox
    • 1
  • Mette Miller
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdense MDenmark