Photosynthesis Research

, Volume 92, Issue 3, pp 389–405

Structure of the Mn4–Ca cluster as derived from X-ray diffraction

  • Jan Kern
  • Jacek Biesiadka
  • Bernhard Loll
  • Wolfram Saenger
  • Athina Zouni
Research Article

DOI: 10.1007/s11120-007-9173-1

Cite this article as:
Kern, J., Biesiadka, J., Loll, B. et al. Photosynth Res (2007) 92: 389. doi:10.1007/s11120-007-9173-1

Abstract

The catalytic centre for light-induced water oxidation in photosystem II (PSII) is a multinuclear metal cluster containing four manganese and one calcium cations. Knowing the structure of this biological catalyst is of utmost importance for unravelling the mechanism of water oxidation in photosynthesis. In this review we describe the current state of the X-ray structure determination at 3.0 Å resolution of the water oxidation complex (WOC) of PSII. The arrangement of metal cations in the cluster, their coordination and protein surroundings are discussed with regard to spectroscopic and mutagenesis studies. Limitations of the presently available structural data are pointed out and possible perspectives for the future are outlined, including the combination of X-ray diffraction and X-ray spectroscopy on single crystals.

Keywords

Mn-clusterPhotosystem IIRadiation damageStructureWater oxidizing complexX-ray absorption spectroscopyX-ray diffraction

Abbreviations

Car

Carotenoid

Chla

Chlorophyll a

ETC

Electron transfer chain

EXAFS

Extended X-ray absorption fine structure

FTIR

Fourier transform infrared

PbRC

Purple bacterial reaction centre

Pheo

Pheophytin a

RC

Reaction centre

PSII

Photosystem II

XAS

X-ray absorption spectroscopy

WOC

Water oxidizing complex

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jan Kern
    • 1
  • Jacek Biesiadka
    • 2
  • Bernhard Loll
    • 2
    • 3
  • Wolfram Saenger
    • 2
  • Athina Zouni
    • 1
  1. 1.Institut für Chemie, Max Volmer Laboratorium für Biophysikalische Chemie, Sekr. PC 14Technische Universität BerlinBerlinGermany
  2. 2.Institut für Chemie und Biochemie/KristallographieFreie Universität BerlinBerlinGermany
  3. 3.Max-Planck-Institut für Medizinische Forschung, Abteilung für Biomolekulare MechanismenHeidelbergGermany