Regular Paper

Photosynthesis Research

, Volume 89, Issue 1, pp 43-48

First online:

Binding of a Transition State Analog to Newly Synthesized Rubisco

  • Boovaraghan BalajiAffiliated withBiology Department, Rensselaer Polytechnic InstituteDivision of Plant Sciences, University of Missouri
  • , Michele GilsonAffiliated withBiology Department, Rensselaer Polytechnic Institute
  • , Harry RoyAffiliated withBiology Department, Rensselaer Polytechnic Institute Email author 

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Radioactive amino acids, when added to isolated pea chloroplasts or chloroplast extracts engaged in protein synthesis, are incorporated into Rubisco large subunits that co-migrate with native Rubisco during nondenaturing electrophoresis. We have added the transition state analog 2′-carboxyarabinitol bisphosphate (CABP) to chloroplast extracts after in organello or in vitro incorporation of radioactive amino acids into Rubisco large subunits. Upon addition of CABP the radioactive bands co-migrating with native Rubisco undergo a readily detected shift in electrophoretic mobility just as the native enzyme, thus demonstrating the ability of the newly assembled molecules to interact with this transition state analog.


Chaperonin Rubisco Protein synthesis Assembly