Plant Molecular Biology Reporter

, Volume 31, Issue 1, pp 222–230

Characterization of the ZmCK1 Gene Encoding a Calcium-Dependent Protein Kinase Responsive to Multiple Abiotic Stresses in Maize

Original Paper

DOI: 10.1007/s11105-012-0496-5

Cite this article as:
Wang, CT. & Shao, JM. Plant Mol Biol Rep (2013) 31: 222. doi:10.1007/s11105-012-0496-5

Abstract

Calcium-dependent protein kinases (CDPKs) play a vital regulatory role in abiotic stress responses in plants. We isolated the ZmCK1 gene encoding a CDPK from maize seedlings. The predicted ZmCK1 protein contains a typical Ser/Thr protein kinase domain and four EF-hand calcium-binding motifs in its N-terminal and C-terminal halves, respectively. The catalytic and regulatory domains were linked by a well-conserved junction domain. A ZmCK1::hGFP fusion protein was found to localize into the cytoplasma and nucleus upon introduction into Arabidopsis mesophyll protoplasts. ZmCK1 transcription was highly activated by salt and cold, and moderately by drought and exogenous ABA in maize seedling. Isolation of the ZmCK1 promoter revealed some cis-acting elements responding to stresses. Overexpression of ZmCK1 improved drought, salt, and cold stress tolerance in transgenic Arabidopsis plants. Our results suggested that ZmCK1 produces a functional kinase that may play a regulatory role in abiotic stress response.

Keywords

CDPK Induction kinetics Abiotic stress Transgenic plants Maize 

Abbreviations

ABA

Abscisic acid

CBL

Calcineurin B-like proteins

CDPK

Calcium-dependent protein kinases

CaM

Calmodulin

MAPKs

Mitogen-activated protein kinases

RLKs

Receptor-like kinases

Q-RT-PCR

Real-time quantitative RT-PCR

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Plant Resources Research and DevelopmentBeijing Technology and Business UniversityBeijingChina
  2. 2.National Key Facility of Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop ScienceChinese Academy of Agriculture Sciences (CAAS)BeijingChina