Plant Molecular Biology Reporter

, Volume 30, Issue 4, pp 973–982

Expression Analysis of Nudix Hydrolase Genes in Chrysanthemum lavandulifolium

Original Paper

DOI: 10.1007/s11105-011-0401-7

Cite this article as:
Huang, H., Cao, H., Niu, Y. et al. Plant Mol Biol Rep (2012) 30: 973. doi:10.1007/s11105-011-0401-7

Abstract

Nudix hydrolases are associated with detoxification processes in plants under abiotic stress and regulate disease resistance signal transduction pathways. Investigating expression patterns and phyletic evolution of eight Nudix hydrolase gene family members in Chrysanthemum lavandulifolium will provide insights into detoxification pathways. In this study, full-length open reading frames of eight Nudix hydrolase genes (ClNUDX1-8) were obtained using a C. lavandulifolium expressed sequence tag (EST) database, EST splicing, and rapid-amplification of cDNA ends. Sequence similarity analysis of the eight ClNUDXs using MEGA4.0 software revealed that these genes belonged to seven subfamilies of the plant Nudix hydrolase gene family. Reverse transcription-polymerase chain reaction analysis demonstrated that five genes exhibited organ-specific expression; these included ClNUDX1, ClNUDX2, ClNUDX3, CINUDX7, and ClNUDX8. Among those, ClNUDX1, ClNUDX3, ClNUDX7, and ClNUDX8 were induced by salt, drought, heat, and cold stresses. ClNUDX2 was only influenced by salt and drought. Transcription levels of both ClNUDX1 and ClNUDX2 were also regulated by abscisic acid and salicylic acid. The promoter of ClNUDX1, CN1P, was cloned. Transient expression analysis indicated that CN1P was capable of driving expression of the GUS reporter gene. Thus, it was concluded that different members of Nudix hydrolase genes in C. lavandulifolium demonstrated varied expression patterns in response to different abiotic stresses. These findings further elucidated knowledge on the response mechanisms to abiotic stresses in C. lavandulifolium.

Keywords

Chrysanthemum lavandulifoliumNudix hydrolasesGene familyPromoter

Abbreviations

ABA

Abscisic acid

ADP

Adenosine 5′-diphosphate

Ap4A

Aiadenosine-5′, 5‴-P1, P4-tetraphosphate

EST

Expressed sequence tag

FAD

Flavin-adeninedinucleotide

GUS

β-glucuronidase

NADPH

Nicotinamide adenine dinucleotide phosphate

NCBI

National Center for Biotechnology Information

NUDX

Nucleoside diphosphates linked to some moiety X

qRT-PCR

Quantitative real-time polymerase chain reaction

RACE

Rapid-amplification of cDNA ends

ROS

Reactive oxygen species

RT-PCR

Reverse transcription-polymerase chain reaction

SA

Salicylic acid

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.College of Landscape ArchitectureBeijing Forestry UniversityBeijingPeople’s Republic of China