Plant Molecular Biology Reporter

, Volume 30, Issue 2, pp 453–461

Determination S-Genotypes and Identification of Five Novel S-RNase Alleles in Wild Malus Species

Authors

    • Laboratory of Fruit Tree Cell and Molecular BreedingChina Agricultural University
  • Shenshan Long
    • Laboratory of Fruit Tree Cell and Molecular BreedingChina Agricultural University
  • Maofu Li
    • Laboratory of Fruit Tree Cell and Molecular BreedingChina Agricultural University
    • Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental BiologyChinese Academy of Sciences
  • Songling Bai
    • United Graduate School of Agricultural ScienceIwate University
    • Laboratory of Fruit Tree Cell and Molecular BreedingChina Agricultural University
Article

DOI: 10.1007/s11105-011-0345-y

Cite this article as:
Li, T., Long, S., Li, M. et al. Plant Mol Biol Rep (2012) 30: 453. doi:10.1007/s11105-011-0345-y

Abstract

Apple (Malus × domestica Borkh.) is a typical Rosaceae species that exhibits gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. In this study, the S-alleles of wild Malus species were amplified, sequenced and compared using polymerase chain reaction (PCR) technology. 21 S-alleles were identified in 27 wild Malus species. The results indicated that the overwhelming majority of S-alleles between wild Malus species and cultivars shared identical sequences. Simultaneously, five new S alleles (designated S48S52) were identified in wild Malus species. There are the S48-RNase in M. angustifolia (Ation) Michaux, S49-RNase in M. orientalis Uglitzk. Ex Juz. and M. sylvestris (L.) Mill., S50-RNase in M. tschonoskii (Maxim.) C.K. Schneid. and M. sieversii (Ldb.) Roem., S51-RNase in M. komarovii (Sarg.) Rehd. and M. kansuensis (Batal.) C. K. Schneid., S52-RNase in M. manshurica (Maxim.) V. Komorov wild Malus species. Additionally, an S1-RNase was cloned in wild Malus prunifolia var. ringo, which have the same open reading frame as Malus × domestica cv. Fuji, but lacked whole intron.

Keywords

Determination and identificationS-GenotypesS-RNaseWild Malus species

Supplementary material

11105_2011_345_MOESM1_ESM.doc (54 kb)
ESM 1(DOC 54 kb)

Copyright information

© Springer-Verlag 2011