Plant Molecular Biology Reporter

, Volume 30, Issue 1, pp 36-45

First online:

A Novel Non-wounding Transient Expression Assay for Cereals Mediated by Agrobacterium tumefaciens

  • Surendar Reddy DhadiAffiliated withDepartment of Biological Sciences, Michigan Technological University
  • , Aparna DeshpandeAffiliated withDepartment of Biological Sciences, Michigan Technological University
  • , Wusirika RamakrishnaAffiliated withDepartment of Biological Sciences, Michigan Technological University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


A novel Agrobacterium tumefaciens-mediated transient expression assay (AmTEA) was developed for young plants of different cereal species and the model dicot Arabidopsis thaliana. AmTEA was evaluated using five promoters (six constructs) and two reporter genes, gus and egfp. The constitutive 35S promoter and the promoter of the rice glutaredoxin gene showed gus and egfp expression in the cereals analyzed in the present study. A promoter for the DEAD-box RNA helicase family protein gene from Arabidopsis showed similar expression patterns of reporter genes in stable transgenic lines as well as in transient expression lines of Arabidopsis. Agrobacterium tumefaciens co-cultivation and plant incubation times were optimized using 35S and the rice expressed protein gene promoter (R2-273). The possibility of non-specific expression of the reporter genes was ruled out by using the antibiotic carbenicillin and the comparison of expression of the reporter genes driven by full-length and truncated R2-273 promoters. AmTEA considerably reduced time, space, labor, and cost requirements. Ease of use with stress treatments is another major advantage of this method. AmTEA can be automated and used for large-scale studies to decipher promoter and gene functions with the ultimate goal to enhance the performance of cereal crops against biotic and abiotic stresses.


Rice Agrobacterium Transient assay Cereal