Plant Molecular Biology Reporter

, Volume 28, Issue 3, pp 460–472

In Silico and Quantitative Analyses of MADS-Box Genes in Coffea arabica

  • Raphael Ricon de Oliveira
  • Antonio Chalfun-Junior
  • Luciano Vilela Paiva
  • Alan Carvalho Andrade
Article

DOI: 10.1007/s11105-009-0173-5

Cite this article as:
de Oliveira, R.R., Chalfun-Junior, A., Paiva, L.V. et al. Plant Mol Biol Rep (2010) 28: 460. doi:10.1007/s11105-009-0173-5

Abstract

MADS-box genes comprise a family of transcription factors that act as key regulators in many cellular development processes of several organisms. Members of this family have highly conserved regions and play important roles as transcription factors, activating target genes. The present work aimed at analyzing the MADS-box gene family present in a database generated by the Brazilian Coffee Genome Project (CAFEST) as well as of observing their respective sites of expression within these data. Through the use of bioinformatics tools, it was possible to identify and classify 26 expressed sequence tag contigs, 13 of them were expressed exclusively in vegetative tissues, 11 in reproductive tissues, and two in both. Later, quantitative analysis by quantitative reverse transcriptase PCR was carried out for three of them belonging to the groups of genes APETALA3 (B genes), AGAMOUS (C genes), and SEPALLATAS (E genes), which could be compared with the expression profile of in silico analysis and of its putative orthologous genes. Therefore, this study aims at a better understanding of the development processes performed by this family of genes in Coffea arabica, mainly in reproductive organs, as well as to compare functions of MADS-box orthologous studied in other species.

Keywords

Coffea arabicaFloral developmentTranscriptomeMADS-box genesqRT-PCR

Supplementary material

11105_2009_173_MOESM1_ESM.doc (46 kb)
Supplementary DataSequence name and GenBank accession numbers of the MADS sequences found in CAFEST (DOC 46 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Raphael Ricon de Oliveira
    • 1
  • Antonio Chalfun-Junior
    • 1
  • Luciano Vilela Paiva
    • 2
  • Alan Carvalho Andrade
    • 3
  1. 1.Department of BiologyFederal University of LavrasMinas GeraisBrazil
  2. 2.Department of ChemistryFederal University of LavrasMinas GeraisBrazil
  3. 3.National Research Center for Genetic Resources and Biotechnology (CENARGEN)Brazilian Company of Agricultural Research (EMBRAPA)BrasiliaBrazil