Plant and Soil

, Volume 347, Issue 1, pp 313–325

Screening of rhizosphere bacteria from grapevine for their suppressive effect on Xiphinema index Thorne & Allen on in vitro grape plants

Regular Article

DOI: 10.1007/s11104-011-0851-6

Cite this article as:
Aballay, E., Mårtensson, A. & Persson, P. Plant Soil (2011) 347: 313. doi:10.1007/s11104-011-0851-6


Xiphinema index is the most aggressive root parasite affecting vines in Chile, controlled mainly chemically. The aim of this study was to identify rhizobacteria in grapevine roots growing in infested soils and to determine if some of these affect the parasitism caused by the nematode. Rhizobacteria of ungrafted grapevine cultivars from 11 vineyards were isolated using TSBA and identified with FAMEs analysis. The antagonistic effect to X. index of those from soils 1 to 4 was assessed in 500 ml pots treating 2 months old in vitro plants with a bacterial suspension containing 1 × 106 c.f.u./ml and 400 nematodes, determining after 4 months growth nematode populations and root damages. Also culture filtrates were assessed. 400 isolates in 25 genera were obtained from the 11 vineyards. The most frequent species were Pseudomonas putida (35.1%), Escherichia coli (7.6%) and Pseudomonas fluorescens (6.1%). Isolates of soils 1 to 4, show that Bacillus megaterium, B. brevis, Pseudomonas corrugata, P. savastanoi, Stenotrophomonas maltophilia and Serratia plymuthica reduced root damage and suppressed populations, meanwhile strains of B. brevis and Comamonas acidovorans increased plant growth but did not control nematodes. It is concluded that these isolates may be useful in biological control programmes in vineyards.


RhizobacteriaVitis viniferaBiological controlPlant-parasitic nematodesRootsXiphinema index



Grape fan leaf virus


Tryptic soy broth agar


Fatty acids methyl esters


Microbial identification system


Redundancy analysis


Analysis of variance


Similarity indices


Plant growth promoting rhizobacteria



Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Erwin Aballay
    • 1
  • Anna Mårtensson
    • 2
  • Paula Persson
    • 3
  1. 1.Department of Crop Protection, Faculty of Agronomical SciencesUniversity of ChileSantiagoChile
  2. 2.Department of Soil and EnvironmentSwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Department of Crop Production EcologySwedish University of Agricultural SciencesUppsalaSweden