Plant and Soil

, Volume 347, Issue 1, pp 313–325

Screening of rhizosphere bacteria from grapevine for their suppressive effect on Xiphinema index Thorne & Allen on in vitro grape plants

Regular Article

DOI: 10.1007/s11104-011-0851-6

Cite this article as:
Aballay, E., Mårtensson, A. & Persson, P. Plant Soil (2011) 347: 313. doi:10.1007/s11104-011-0851-6

Abstract

Xiphinema index is the most aggressive root parasite affecting vines in Chile, controlled mainly chemically. The aim of this study was to identify rhizobacteria in grapevine roots growing in infested soils and to determine if some of these affect the parasitism caused by the nematode. Rhizobacteria of ungrafted grapevine cultivars from 11 vineyards were isolated using TSBA and identified with FAMEs analysis. The antagonistic effect to X. index of those from soils 1 to 4 was assessed in 500 ml pots treating 2 months old in vitro plants with a bacterial suspension containing 1 × 106 c.f.u./ml and 400 nematodes, determining after 4 months growth nematode populations and root damages. Also culture filtrates were assessed. 400 isolates in 25 genera were obtained from the 11 vineyards. The most frequent species were Pseudomonas putida (35.1%), Escherichia coli (7.6%) and Pseudomonas fluorescens (6.1%). Isolates of soils 1 to 4, show that Bacillus megaterium, B. brevis, Pseudomonas corrugata, P. savastanoi, Stenotrophomonas maltophilia and Serratia plymuthica reduced root damage and suppressed populations, meanwhile strains of B. brevis and Comamonas acidovorans increased plant growth but did not control nematodes. It is concluded that these isolates may be useful in biological control programmes in vineyards.

Keywords

RhizobacteriaVitis viniferaBiological controlPlant-parasitic nematodesRootsXiphinema index

Abbreviations

GFLV

Grape fan leaf virus

TSBA

Tryptic soy broth agar

FAMEs

Fatty acids methyl esters

MIS

Microbial identification system

RDA

Redundancy analysis

ANOVA

Analysis of variance

SIs

Similarity indices

PGPR

Plant growth promoting rhizobacteria

ACC

1-Aminocyclopropane-1-carboxylate

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Erwin Aballay
    • 1
  • Anna Mårtensson
    • 2
  • Paula Persson
    • 3
  1. 1.Department of Crop Protection, Faculty of Agronomical SciencesUniversity of ChileSantiagoChile
  2. 2.Department of Soil and EnvironmentSwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Department of Crop Production EcologySwedish University of Agricultural SciencesUppsalaSweden