Plant and Soil

, Volume 339, Issue 1, pp 231–245

Traits related to differences in function among three arbuscular mycorrhizal fungi

  • Cécile Thonar
  • Andrea Schnepf
  • Emmanuel Frossard
  • Tiina Roose
  • Jan Jansa
Regular Article

DOI: 10.1007/s11104-010-0571-3

Cite this article as:
Thonar, C., Schnepf, A., Frossard, E. et al. Plant Soil (2011) 339: 231. doi:10.1007/s11104-010-0571-3

Abstract

Diversity in phosphorus (P) acquisition strategies was assessed among three species of arbuscular mycorrhizal fungi (AMF) isolated from a single field in Switzerland. Medicago truncatula was used as a test plant. It was grown in a compartmented system with root and root-free zones separated by a fine mesh. Dual radioisotope labeling (32P and 33P) was employed in the root-free zone as follows: 33P labeling determined hyphal P uptake from different distances from roots over the entire growth period, whereas 32P labeling investigated hyphal P uptake close to the roots over the 48 hours immediately prior to harvest. Glomus intraradices, Glomus claroideum and Gigaspora margarita were able to take up and deliver P to the plants from maximal distances of 10, 6 and 1 cm from the roots, respectively. Glomus intraradices most rapidly colonized the available substrate and transported significant amounts of P towards the roots, but provided the same growth benefit as compared to Glomus claroideum, whose mycelium was less efficient in soil exploration and in P uptake and delivery to the roots. These differences are probably related to different carbon requirements by these different Glomus species. Gigaspora margarita provided low P benefits to the plants and formed dense mycelium networks close to the roots where P was probably transiently immobilized. Numerical modeling identified possible mechanisms underlying the observed differences in patterns of mycelium growth. High external hyphal production at the root-fungus interface together with rapid hyphal turnover were pointed out as important factors governing hyphal network development by Gigaspora, whereas nonlinearity in apical branching and hyphal anastomoses were key features for G. intraradices and G. claroideum, respectively.

Keywords

Arbuscular mycorrhiza Extraradical mycelium Functional diversity Hyphal growth model Medicago truncatula Phosphorus 

Abbreviations

AMF

Arbuscular mycorrhizal fungi

ANOVA

Analysis of variance

DW

Dry weight

ERM

Extraradical mycelium

HLD

Hyphal length density

NM

Non-mycorrhizal

P

Phosphorus

p

Probability level

PUE

Phosphorus Use Efficiency

RMSE

Root mean squared error

Supplementary material

11104_2010_571_MOESM1_ESM.pdf (254 kb)
Online resource 1(PDF 253 kb)
11104_2010_571_MOESM2_ESM.pdf (319 kb)
Online resource 2(PDF 318 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Cécile Thonar
    • 1
    • 2
    • 5
  • Andrea Schnepf
    • 3
  • Emmanuel Frossard
    • 1
  • Tiina Roose
    • 4
  • Jan Jansa
    • 1
  1. 1.ETH Zurich, Institute of Plant, Animal and Agroecosystem Sciences, Plant Nutrition GroupLindauSwitzerland
  2. 2.CIAT-TSBF, Tropical Soil Biology & Fertility InstituteNairobiKenya
  3. 3.Department of Forest and Soil Sciences, Institute of Soil ScienceBOKU-University of Natural Resources and Applied Life SciencesViennaAustria
  4. 4.School of Engineering SciencesUniversity of SouthamptonSouthamptonUK
  5. 5.ETH Zurich, Plant SciencesLindauSwitzerland

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