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Herbicide application increases nitrogen (15N) exudation and root detachment of Brachiaria decumbens Stapf

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Abstract

Current research shows that glyphosate herbicides can increase nitrogen exudation by plant roots and, as a consequence, favor pathogenic fungal colonization in soil. In Brazilian agroecosystems, the main herbicides used in conservationist systems for glyphosate substitution are glufosinate and paraquat. However, no studies have been developed that to evaluate the effects of these herbicides on nitrogen exudation by roots. This work was carried out with the objective of evaluating the exudation of nitrogen (15N) compounds and root detachment after the application of glyphosate, glufosinate-ammonium or paraquat on Brachiaria decumbens Stapf. The ammonium concentration in the plant’s tissues and the nitrogen (15N) recovery in the plant-solution system were also evaluated. The nitrogen left by the soil-solution system and the ammonium concentration in the plant’s tissues were increased after glyphosate or glufosinate application, but they were not modified by paraquat. Nitrogen compound exudation and root detachment were increased by the desiccation of Brachiaria decumbens Stapf by the use of the following herbicides: glyphosate, glufosinate-ammonium and paraquat. This increase demonstrated that the nitrogen exudation was not affected by a particular herbicide’s mode of action.

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References

  • Aulakh MS, Wassmann R, Bueno C, Kreuzwiser J, Rennenberg H (2001) Characterization of root exudates at different growth stages of ten rice (Oryza sativa L.) cultivars. Plant biol 3:139–148

    Article  CAS  Google Scholar 

  • Awad AE, Worsham AD, Corbin FT, Eplee RE (1991) Absorption, translocation and metabolism of foliarly applied 14C-dicamba in sorghum (Sorghum bicolor) and corn (Zea mays) parasitized with witchweed (Striga asiatica). In: Proceedings 1991 15th International Symposium on Parasitic Weeds, Nairobi, Kenya, 535–536

  • Badri DV, Vivanco JM (2009) Regulation and function of root exudates. Plant Soil Environ 32:661–681

    Google Scholar 

  • Barrie A, Prosser SJ (1996) Automated analysis of light-element stable isotopes by isotope ratio mass spectrometry. In: Boutton TW, Yamsahi S (eds) Mass spectrometry of soils. Dekker, New York, pp 1–46

    Google Scholar 

  • Bremer Neto H, Victoria Filho R, Mourão Filho FAA, Menezes GM, Canalli E (2008) Nutritional status and production of ‘Pêra’ sweet orange related to cover crops and mulch. Pesq Agropec Bras 43:29–35

    Article  Google Scholar 

  • Brophy LS, Heichel GH (1989) Nitrogen release from roots of alfafa and soybean grown in sand culture. Plant soil 116:77–84

    Article  CAS  Google Scholar 

  • Dakora FD, Phillips DA (2002) Roots exudates as mediators of mineral acquisition in low-nutrient environments. Plant Soil 245:35–47

    Article  CAS  Google Scholar 

  • Damin V, Franco HCJ, Moraes MF, Franco A, Trivelin PCO (2008) Nitrogen loss in Brachiaria decumbens after application of glyphosate or glufosinate-ammonium. Sci Agric 65:402–407

    Article  CAS  Google Scholar 

  • Damin V, Trivelin PCO, Franco HJF, Barbosa TG (2009) Nitrogen(15N) loss in the soil–plant system after herbicide application on Pennisetum glaucum. Plant Soil. doi:10.1007/s11104-009-0106-y

    Google Scholar 

  • Deubel A, Gransee A, Merbach W (2000) Transformation of organic rhizodephositions by rhizosfere bacteria and its influence on the availability of tertiary calcium phosphate. J Plant Nutr Soil Sci 163:387–392

    Article  CAS  Google Scholar 

  • Duke SO, Hoagland RE (1985) Effects of glyphosate on metabolism of phenolic compounds. In: Grossbard E, Atkinson D (eds) The herbicide glyphosate. Butterworths, London, pp 75–91

    Google Scholar 

  • Epstein E, Bloom AJ (2005) Mineral nutrition of plants: principles and perspectives, 2nd edn. Sinauer, Sunderland, p 400

    Google Scholar 

  • Fernandez CJ, Mcinnes KJ, Cothren JT (1994) Carbon balance, transpiration and biomass partitioning of glyphosate-treated wheat (Triticum aestivum) plants. Weed Sci 42:333–339

    CAS  Google Scholar 

  • Garcia JAL, Barbas C, Probanza A, Barrientos ML, Manero FJC (2001) Low molecular weight organic acids and fatty acids in root exudates of two Lupinus cultivars at flowering and fruiting stages. Phytochem Anal 12:305–311

    Article  CAS  Google Scholar 

  • Gubbiga NG, Worsham AD, Corbin FT (1996) Root/rhizome exudation of nicosulfuron from treated johnsongrass (Sorghum halepense) and possible implications for corn (Zea mays). Weed Sci 44:455–460

    CAS  Google Scholar 

  • Henry S, Texier S, Hallet S, Bru D, Dambreville C, Cheneby D, Bizouard F, Germon JC, Phillippot L (2008) Disentangling the rhizosphere effce on nitrate reducers and denitrifiers: insight into the role of root exudates. Environ Microbiol 11:3082–3092

    Article  CAS  Google Scholar 

  • Hoagland DR, Arnon DI (1950) The water culture method of growing plants without soil. University of California, Berkeley, p 32

    Google Scholar 

  • Holland JN, Cheng W, Crossley DAJ (1996) Herbivore induced changes in plant carbon allocation assessment of below-ground C fluxes using carbon-14. Oecology 107:87–94

    Article  Google Scholar 

  • Keith H, Oades JM, Martin JK (1986) Input of carbon to soil from wheat plants. Soil Biol Biochem 18:445–449

    Article  CAS  Google Scholar 

  • Klein DA, Frederick BA, Biondini MJT (1988) Rhizosphere microorganisms effects on soluble amino acids, sugars and organic acids in the root zone of Agropyron cristatum, A. smithii and Bouteloua gracilis. Plant Soil 110:19–25

    Article  CAS  Google Scholar 

  • Kraffczyk I, Trolldenier G, Beringer H (1984) Soluble root exudates of maize (Zea mays L.): influence of potassium supply and rhizosfere microorganisms. Soil Biol Biochem 16:315–322

    Article  CAS  Google Scholar 

  • Kremer RJ, Means NE, Kim S (2005) Glyphosate affects soybean root exudation and rhizosphere microorganisms. Intern J Environ Anal Chem 85:1165–1174

    Article  CAS  Google Scholar 

  • Liu L, Punja ZK, Rahe JE (1997) Altered root exudation and suppression of induced lignification as mechanisms of predisposition by glyphosate of bean roots (Phaseolus vulgaris L.) to colonization by Pythium spp. Physiol Mol Plant Pathol 51:111–127

    Article  CAS  Google Scholar 

  • Manderscheid R, Schaaf S, Mattsson M, Schjoerring JK (2005) Glufosinate treatment of weeds results in ammonia emission by plants. Agric Ecosys Environ 109:129–140

    Article  CAS  Google Scholar 

  • Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London, p 889

    Google Scholar 

  • Merbach W, Schlze J, Richert M, Rrocco E, Mengel K (2003) A comparison of different 15N application techniques to study the net rhizodeposition in the plant-soil system. J Plant Nutr Soil Sci 163:375–379

    Article  Google Scholar 

  • Mijangos I, Becerril JM, Albizu I, Epelde L, Garbisu C (2009) Effects of glyphosate on rhizosphere soil microbial communities under two different plant compositions by cultivation-dependent and -independent methodologies. Soil Biol Biochem 41:505–513

    Article  CAS  Google Scholar 

  • Nguyen C (2003) Rhizodeposition of organic C by plant, mechanisms and controls. Agronomie 23:375–396

    Article  CAS  Google Scholar 

  • Ofuso-Budu KG, Fujita K, Ogata S (1990) Excretion ureide and other nitrogenous compounds by the root system of soybean at different growth stages. Plant Soil 128:135–142

    Article  Google Scholar 

  • Paterson E (2003) Importance of rhizodeposition in the coupling of plant and microbial productivity. European J Soil Sci 54:741–750

    Article  Google Scholar 

  • Paynel F, Murray PJ, Cliquet JB (2001) Root exudates, a pathway for short-term transfer from clover and ryegrass. Plant Soil 229:235–243

    Article  CAS  Google Scholar 

  • Reis BF, Vieira JA, Krug FJ, Giné MF (1997) Development of a flow injections system two analytical paths for ammonium determination in soil extracts by conductometry. J Brazilian Chem Soc 8:523–528

    CAS  Google Scholar 

  • Schjoerring JK, Husted S, Mattsson M (1998) Physiological parameters controlling plant-atmospheric ammonia exchange. Atmosferic Environ 32:491–498

    Article  CAS  Google Scholar 

  • Sørensen J (1997) The rhizosphere as a habitat for soil microorganisms. In: Elsas JDV, Trevors JT, Wellington EMK (eds) Modern soil microbiology. Dekker, New York

    Google Scholar 

  • Tiedje JM (1988) Ecology of denitrification and dissimilatory nitrate reduction to ammonium. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 179–244

    Google Scholar 

  • Tuffi Santos LD, Santos JB, Ferreira FA, Oliveira JA, Bentivenha S, Machado AFL (2008) Radicular exudation of glyphosate by Brachiaria decumbens and its effects on eucalypt plant. Weed 26:369–374

    Google Scholar 

  • Xia JH, Roberts JKM (1994) Improved cytoplasmic pH regulation lactate levels are biochemical traits expressed in root tips of whole maize seedlings acclimated to a low oxygen environmental. Plant Physiol 105:651–657

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

To CNPq for scholarships granted to the authors and to FAPESP for supporting the project.

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Correspondence to Virginia Damin.

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Responsible Editor: Ismail Cakmak.

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Damin, V., Trivelin, P.C.O., Carvalho, S.J.P. et al. Herbicide application increases nitrogen (15N) exudation and root detachment of Brachiaria decumbens Stapf. Plant Soil 334, 511–519 (2010). https://doi.org/10.1007/s11104-010-0402-6

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