Abstract
Current research shows that glyphosate herbicides can increase nitrogen exudation by plant roots and, as a consequence, favor pathogenic fungal colonization in soil. In Brazilian agroecosystems, the main herbicides used in conservationist systems for glyphosate substitution are glufosinate and paraquat. However, no studies have been developed that to evaluate the effects of these herbicides on nitrogen exudation by roots. This work was carried out with the objective of evaluating the exudation of nitrogen (15N) compounds and root detachment after the application of glyphosate, glufosinate-ammonium or paraquat on Brachiaria decumbens Stapf. The ammonium concentration in the plant’s tissues and the nitrogen (15N) recovery in the plant-solution system were also evaluated. The nitrogen left by the soil-solution system and the ammonium concentration in the plant’s tissues were increased after glyphosate or glufosinate application, but they were not modified by paraquat. Nitrogen compound exudation and root detachment were increased by the desiccation of Brachiaria decumbens Stapf by the use of the following herbicides: glyphosate, glufosinate-ammonium and paraquat. This increase demonstrated that the nitrogen exudation was not affected by a particular herbicide’s mode of action.
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References
Aulakh MS, Wassmann R, Bueno C, Kreuzwiser J, Rennenberg H (2001) Characterization of root exudates at different growth stages of ten rice (Oryza sativa L.) cultivars. Plant biol 3:139–148
Awad AE, Worsham AD, Corbin FT, Eplee RE (1991) Absorption, translocation and metabolism of foliarly applied 14C-dicamba in sorghum (Sorghum bicolor) and corn (Zea mays) parasitized with witchweed (Striga asiatica). In: Proceedings 1991 15th International Symposium on Parasitic Weeds, Nairobi, Kenya, 535–536
Badri DV, Vivanco JM (2009) Regulation and function of root exudates. Plant Soil Environ 32:661–681
Barrie A, Prosser SJ (1996) Automated analysis of light-element stable isotopes by isotope ratio mass spectrometry. In: Boutton TW, Yamsahi S (eds) Mass spectrometry of soils. Dekker, New York, pp 1–46
Bremer Neto H, Victoria Filho R, Mourão Filho FAA, Menezes GM, Canalli E (2008) Nutritional status and production of ‘Pêra’ sweet orange related to cover crops and mulch. Pesq Agropec Bras 43:29–35
Brophy LS, Heichel GH (1989) Nitrogen release from roots of alfafa and soybean grown in sand culture. Plant soil 116:77–84
Dakora FD, Phillips DA (2002) Roots exudates as mediators of mineral acquisition in low-nutrient environments. Plant Soil 245:35–47
Damin V, Franco HCJ, Moraes MF, Franco A, Trivelin PCO (2008) Nitrogen loss in Brachiaria decumbens after application of glyphosate or glufosinate-ammonium. Sci Agric 65:402–407
Damin V, Trivelin PCO, Franco HJF, Barbosa TG (2009) Nitrogen(15N) loss in the soil–plant system after herbicide application on Pennisetum glaucum. Plant Soil. doi:10.1007/s11104-009-0106-y
Deubel A, Gransee A, Merbach W (2000) Transformation of organic rhizodephositions by rhizosfere bacteria and its influence on the availability of tertiary calcium phosphate. J Plant Nutr Soil Sci 163:387–392
Duke SO, Hoagland RE (1985) Effects of glyphosate on metabolism of phenolic compounds. In: Grossbard E, Atkinson D (eds) The herbicide glyphosate. Butterworths, London, pp 75–91
Epstein E, Bloom AJ (2005) Mineral nutrition of plants: principles and perspectives, 2nd edn. Sinauer, Sunderland, p 400
Fernandez CJ, Mcinnes KJ, Cothren JT (1994) Carbon balance, transpiration and biomass partitioning of glyphosate-treated wheat (Triticum aestivum) plants. Weed Sci 42:333–339
Garcia JAL, Barbas C, Probanza A, Barrientos ML, Manero FJC (2001) Low molecular weight organic acids and fatty acids in root exudates of two Lupinus cultivars at flowering and fruiting stages. Phytochem Anal 12:305–311
Gubbiga NG, Worsham AD, Corbin FT (1996) Root/rhizome exudation of nicosulfuron from treated johnsongrass (Sorghum halepense) and possible implications for corn (Zea mays). Weed Sci 44:455–460
Henry S, Texier S, Hallet S, Bru D, Dambreville C, Cheneby D, Bizouard F, Germon JC, Phillippot L (2008) Disentangling the rhizosphere effce on nitrate reducers and denitrifiers: insight into the role of root exudates. Environ Microbiol 11:3082–3092
Hoagland DR, Arnon DI (1950) The water culture method of growing plants without soil. University of California, Berkeley, p 32
Holland JN, Cheng W, Crossley DAJ (1996) Herbivore induced changes in plant carbon allocation assessment of below-ground C fluxes using carbon-14. Oecology 107:87–94
Keith H, Oades JM, Martin JK (1986) Input of carbon to soil from wheat plants. Soil Biol Biochem 18:445–449
Klein DA, Frederick BA, Biondini MJT (1988) Rhizosphere microorganisms effects on soluble amino acids, sugars and organic acids in the root zone of Agropyron cristatum, A. smithii and Bouteloua gracilis. Plant Soil 110:19–25
Kraffczyk I, Trolldenier G, Beringer H (1984) Soluble root exudates of maize (Zea mays L.): influence of potassium supply and rhizosfere microorganisms. Soil Biol Biochem 16:315–322
Kremer RJ, Means NE, Kim S (2005) Glyphosate affects soybean root exudation and rhizosphere microorganisms. Intern J Environ Anal Chem 85:1165–1174
Liu L, Punja ZK, Rahe JE (1997) Altered root exudation and suppression of induced lignification as mechanisms of predisposition by glyphosate of bean roots (Phaseolus vulgaris L.) to colonization by Pythium spp. Physiol Mol Plant Pathol 51:111–127
Manderscheid R, Schaaf S, Mattsson M, Schjoerring JK (2005) Glufosinate treatment of weeds results in ammonia emission by plants. Agric Ecosys Environ 109:129–140
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic, London, p 889
Merbach W, Schlze J, Richert M, Rrocco E, Mengel K (2003) A comparison of different 15N application techniques to study the net rhizodeposition in the plant-soil system. J Plant Nutr Soil Sci 163:375–379
Mijangos I, Becerril JM, Albizu I, Epelde L, Garbisu C (2009) Effects of glyphosate on rhizosphere soil microbial communities under two different plant compositions by cultivation-dependent and -independent methodologies. Soil Biol Biochem 41:505–513
Nguyen C (2003) Rhizodeposition of organic C by plant, mechanisms and controls. Agronomie 23:375–396
Ofuso-Budu KG, Fujita K, Ogata S (1990) Excretion ureide and other nitrogenous compounds by the root system of soybean at different growth stages. Plant Soil 128:135–142
Paterson E (2003) Importance of rhizodeposition in the coupling of plant and microbial productivity. European J Soil Sci 54:741–750
Paynel F, Murray PJ, Cliquet JB (2001) Root exudates, a pathway for short-term transfer from clover and ryegrass. Plant Soil 229:235–243
Reis BF, Vieira JA, Krug FJ, Giné MF (1997) Development of a flow injections system two analytical paths for ammonium determination in soil extracts by conductometry. J Brazilian Chem Soc 8:523–528
Schjoerring JK, Husted S, Mattsson M (1998) Physiological parameters controlling plant-atmospheric ammonia exchange. Atmosferic Environ 32:491–498
Sørensen J (1997) The rhizosphere as a habitat for soil microorganisms. In: Elsas JDV, Trevors JT, Wellington EMK (eds) Modern soil microbiology. Dekker, New York
Tiedje JM (1988) Ecology of denitrification and dissimilatory nitrate reduction to ammonium. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 179–244
Tuffi Santos LD, Santos JB, Ferreira FA, Oliveira JA, Bentivenha S, Machado AFL (2008) Radicular exudation of glyphosate by Brachiaria decumbens and its effects on eucalypt plant. Weed 26:369–374
Xia JH, Roberts JKM (1994) Improved cytoplasmic pH regulation lactate levels are biochemical traits expressed in root tips of whole maize seedlings acclimated to a low oxygen environmental. Plant Physiol 105:651–657
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To CNPq for scholarships granted to the authors and to FAPESP for supporting the project.
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Damin, V., Trivelin, P.C.O., Carvalho, S.J.P. et al. Herbicide application increases nitrogen (15N) exudation and root detachment of Brachiaria decumbens Stapf. Plant Soil 334, 511–519 (2010). https://doi.org/10.1007/s11104-010-0402-6
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DOI: https://doi.org/10.1007/s11104-010-0402-6