Plant and Soil

, Volume 319, Issue 1, pp 307–321

A 1H NMR study of water flow in Phaseolus vulgaris L. roots treated with nitrate or ammonium

  • Thomas Schulze-Till
  • Ilja Kaufmann
  • Burkhard Sattelmacher
  • Peter Jakob
  • Axel Haase
  • Shiwei Guo
  • Ulrich Zimmermann
  • Lars H. Wegner
Regular Article

DOI: 10.1007/s11104-008-9871-2

Cite this article as:
Schulze-Till, T., Kaufmann, I., Sattelmacher, B. et al. Plant Soil (2009) 319: 307. doi:10.1007/s11104-008-9871-2

Abstract

The effect of N nutrition on root xylem (water) flow in Phaseolus vulgaris was studied by using the 1H NMR flow imaging technique. Plants pre-cultivated on CaSO4-solution were transferred to NMR-compatible split-root cuvettes filled with half-strength nitrate medium (2.5 mM). After 6 days full-strength (5 mM) nitrate medium was supplied to both compartments (NN treatment), or only to one compartment, whereas the other one was perfused with full-strength (5 mM) ammonium medium (NA treatment). Imaging was performed 7–8 days later. Root growth, xylem flow velocity and xylem pressure (measured with the xylem pressure probe) were largely unaffected by N nutrition. Under NA conditions, volume flow was significantly lower in ammonium- compared to nitrate-fed roots due to a reduced number of functional xylem elements. This was apparently not related to anatomical differences nor to an enhanced number of cavitated vessels in ammonium-fed roots. Vessels in these roots may become dysfunctional by clogging to prevent cavitation.

Keywords

Phaseolus vulgaris (French bean)1H NMR flow imagingSplit-root setupNitrateAmmoniumPlant water relationsXylem pressure

Abbreviations

DW

Dry weight

NA

Under split-root conditions, roots are either supplied with nitrate or ammonium nutrient solution

NN

Under split-root conditions, all roots are supplied with the same nitrate nutrient solution

NMR

Nuclear magnetic resonance

Px

Xylem pressure

TSE

Turbo-spin-echo

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Thomas Schulze-Till
    • 1
  • Ilja Kaufmann
    • 1
  • Burkhard Sattelmacher
    • 2
  • Peter Jakob
    • 1
  • Axel Haase
    • 1
  • Shiwei Guo
    • 2
    • 4
  • Ulrich Zimmermann
    • 3
  • Lars H. Wegner
    • 3
    • 5
  1. 1.Lehrstuhl für Experimentelle Physik VUniversität WürzburgWürzburgGermany
  2. 2.Institut für Pflanzenernährung und BodenkundeUniversität KielKielGermany
  3. 3.Lehrstuhl für Biotechnologie, BiozentrumUniversität WürzburgWürzburgGermany
  4. 4.College of Natural Resources and Environmental SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
  5. 5.Plant Bioelectrics GroupKarlsruhe Institute of TechnologyEggenstein-LeopoldshafenGermany