Plant and Soil

, Volume 297, Issue 1, pp 105–118

Identification of new sources of aluminum resistance in wheat

  • Li-Li Zhou
  • Gui-Hua Bai
  • Brett Carver
  • Da-Dong Zhang
Regular Article

DOI: 10.1007/s11104-007-9324-3

Cite this article as:
Zhou, LL., Bai, GH., Carver, B. et al. Plant Soil (2007) 297: 105. doi:10.1007/s11104-007-9324-3

Abstract

Aluminum (Al) toxicity is a major constraint for wheat production in acidic soils. An Al resistance gene on chromosome 4DL that traces to Brazilian wheat has been extensively studied, and can provide partial protection from Al damage. To identify potentially new sources of Al resistance, 590 wheat accessions, including elite wheat breeding lines from the United States and other American and European countries, landraces and commercial cultivars from East Asia, and synthetic wheat lines from CIMMYT, Mexico, were screened for Al resistance by measuring relative root elongation in culture with a nutrient solution containing Al, and by staining Al-stressed root tips with hematoxylin. Eighty-eight wheat accessions demonstrated at least moderate resistance to Al toxicity. Those selected lines were subjected to analysis of microsatellite markers linked to an Al resistance gene on 4DL and a gene marker for the Al-activated malate transporter (ALMT1) locus. Many of the selected Al-resistant accessions from East Asia did not have the Al-resistant marker alleles of ALMT1, although they showed Al resistance similar to the US Al-resistant cultivar, Atlas 66. Most of the cultivars derived from Jagger and Atlas 66 have the Al-resistant marker alleles of ALMT1. Cluster analysis separated the selected Al-resistant germplasm into two major clusters, labeled as Asian and American–European clusters. Potentially new germplasm of Al resistance different from those derived from Brazil were identified. Further investigation of Al resistance in those new germplasms may reveal alternative Al-resistance mechanisms in wheat.

Keywords

Triticum aestivumAl resistanceMarker-assisted selectionAcidic soil

Abbreviations

Al

aluminum

RRG

relative root growth

NRG

net root growth

HSS

hematoxylin staining score

ALMT

Al-induced malate transporter

SSR

simple sequence repeat

Supplementary material

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Li-Li Zhou
    • 1
  • Gui-Hua Bai
    • 2
  • Brett Carver
    • 3
  • Da-Dong Zhang
    • 1
  1. 1.Department of AgronomyKansas State UniversityManhattanUSA
  2. 2.USDA-ARS Plant Science and Entomology Research UnitManhattanUSA
  3. 3.Department of Plant and Soil SciencesOklahoma State UniversityStillwaterUSA