Plant and Soil

, Volume 290, Issue 1, pp 343–355

Nitrogen nutrition influences some biochemical responses to iron deficiency in tolerant and sensitive genotypes of Vitis

  • S. Jiménez
  • Y. Gogorcena
  • C. Hévin
  • A. D. Rombolà
  • N. Ollat
Original Paper

DOI: 10.1007/s11104-006-9166-4

Cite this article as:
Jiménez, S., Gogorcena, Y., Hévin, C. et al. Plant Soil (2007) 290: 343. doi:10.1007/s11104-006-9166-4

Abstract

The effects of nitrogen source on iron deficiency responses were investigated in two Vitis genotypes, one tolerant to limestone chlorosis Cabernet Sauvignon (Vitis vinifera cv.) and the other susceptible Gloire de Montpellier (Vitis riparia cv.). Plants were grown with or without Fe(III)-EDTA, and with NO3 alone or a mixture of NO3 and NH4+. Changes in pH of the nutrient solution and root ferric chelate reductase (FC-R) activity were monitored over one week. We carried out quantitative metabolic profiling (1H-NMR) and determined the activity of enzymes involved in organic acid metabolism in root tips. In iron free-solutions, with NO3 as the sole nitrogen source, the typical Fe-deficiency response reactions as acidification of the growth medium and enhanced FC-R activity in the roots were observed only in the tolerant genotype. Under the same nutritional conditions, organic acid accumulation (mainly citrate and malate) was found for both genotypes. In the presence of NH4+, the sensitive genotype displayed some decrease in pH of the growth medium and an increase in FC-R activity. For both genotypes, the presence of NH4+ ions decreased significantly the organic acid content of roots. Both Vitis genotypes were able to take up NH4+ from the nutrient solution, regardless of their sensitivity to iron deficiency. The presence of N-NH4+ modified typical Fe stress responses in tolerant and sensitive Vitis genotypes.

Keywords

AmmoniumFerric chelate reductaseGrapevineOrganic acidsRhizosphere acidificationRootstocks

Abbreviations

2OG

2-oxoglutarate

BPDS

bathophenanthrolinedisulfonic acid disodium salt hydrate

BSA

bovine serum albumin

CoA

coenzyme A

DEA

diethanolamine

DTNB

5-5′-dithio-bis-2-nitrobenzoic acid

DTT

DL-dithiothreitol

EDTA

ethylenediaminetetracetic acid

ERETIC

electronic reference to access in vivo concentrations

FW

fresh weight

GOGAT

glutamate synthetase

GS

glutamine synthetase

MDH

malate dehydrogenase

MES

2-(N-morpholino)ethanesulfonic acid

NMR

nuclear magnetic resonance

PEP

phosphoenolpyruvate

PEPC

phosphoenolpyruvate carboxylase

PMSF

phenylmethylsulfonyl fluoride

PVPP

polyvinylpolypyrrolidone

TCA

tricarboxylic acid

TEA

triethylamine

TSP

(trimethyl) propionic-2,3,3,3-d4 acid sodium salt

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • S. Jiménez
    • 1
  • Y. Gogorcena
    • 1
  • C. Hévin
    • 2
  • A. D. Rombolà
    • 3
  • N. Ollat
    • 2
  1. 1.Departamento de PomologíaEstación Experimental de Aula Dei, CSICZaragozaSpain
  2. 2.Unité de Recherches sur les Espèces Fruitières et la VigneINRAVillenave d’OrnonFrance
  3. 3.Dipartimento di Colture ArboreeUniversità di BolognaBolognaItaly